持续性角膜上皮缺损对骨髓间充质干细胞增殖能力的影响  

Effects of persistent corneal epithelial defect on proliferation capacity of bone marrow mesenchymal stem cell

作　　者：齐文娟[1] 吴静[2] 侯光辉[3] 王观峰[1] 陈剑[1] 徐锦堂[2] 

机构地区：[1]暨南大学附属第一医院眼科,广东省广州市510632 [2]暨南大学医学院眼科研究室,广东省广州市510632 [3]珠海市人民医院眼科,广东省珠海市519000

出　　处：《眼科新进展》2012年第8期701-704,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30872808);珠海市科技计划项目基金资助(编号:PB20051014);暨南大学侨办重点学科基金资助(编号:51205004)~~

摘　　要：目的建立持续性角膜上皮缺损模型,观察不同时相的角膜上皮缺损对骨髓间充质干细胞增殖能力的影响。方法取健康雄性Wistar大鼠40只,随机平均分为8组,按每天处死1组大鼠的先后顺序分别命名为缺损组(A、B、C、D、E、F、G)和空白对照组。对全部缺损组大鼠建立持续性角膜上皮缺损模型,每天对缺损组大鼠左眼行角膜上皮刮除术,第1次术后1d处死缺损组A组大鼠,然后对剩余缺损组大鼠左眼行角膜上皮刮除术;第2次术后1d处死缺损组B组大鼠,然后对剩余缺损组大鼠左眼行角膜上皮刮除术,依此类推连续7d,并在无菌条件下获取各组骨髓间充质干细胞,流式细胞技术检测各组细胞周期。结果骨髓间充质干细胞细胞周期检测结果显示:B、D组S期细胞百分数分别为16.76%±4.49%、17.95%±2.77%,明显高于其他缺损组和对照组(均为P<0.05),B、D组G1期细胞百分数分别为72.26%±8.81%、71.33%±5.30%,均低于其他缺损组和对照组(均为P<0.05);但B、D组S期细胞及G1期细胞百分数差异无统计学意义(均为P>0.05),A、C、E、F、G组及对照组间的S期细胞及G1期细胞百分数差异亦无统计学意义(均为P>0.05)。结论持续性角膜上皮缺损模型,在缺损持续的第3天和第5天骨髓间充质干细胞的增殖能力明显提高。Objective To establish a persistent corneal epithelial defect model,and observe the effects of corneal epithelial defects on the proliferation capacity of bone marrow mesenchymal stem cells（BMSC）.Methods Forty healthy male Wistar rats were randomly divided into 8 groups,according to the turns by which they were put to death,and named as defect groups（A,B,C,D,E,F,G） and control group.The persistent corneal epithelial defect model was made in all the defect groups.The same area corneal epithelium was removed every day in the left eye of all defect groups,the following day after first surgery,the rats of defect group A were put to death,and the following day after second surgery,the rats of defect group B were put to death,the rest defect groups were did like that,and the BMSC were isolated and cultivated under aseptic conditions,the changes of cell cycle were examined by flow cytometry.Results From the results of the cell cycle examination,compared with other defect groups and control group,the portions of S-phase BMSC in group B and D were 16.76%±4.49% and 17.95%±2.77%,obviously higher than those in other defect groups and control group（both P0.05）,and also no statistical difference among group A,C,E,F,G and control group（both P〉0.05）.Conclusion The experiment has designed a persistent corneal epithelial defect model,the proliferation capacity of BMSC is significantly improved on day 3 and 5 during the consecutive process of corneal epithelial defect.

关 键 词：欁骨髓间充质干细胞 细胞周期 角膜上皮缺损 动物模型 

分 类 号：R772.205[医药卫生—眼科]