单次延长应激导致的创伤后应激障碍行为伴随乳头体神经元活化  

The change of c-fos expression in mammillary body after single prolonged stress

作　　者：刘俊丽[1] 夏亮[2] 王莉颖[3] 田菲[4] 翟明珠[3] 鲁亚成[3] 李云庆[3] 朱霞[2] 王文[3] 

机构地区：[1]西安工业大学思政部心理健康教育中心,西安710021 [2]第四军医大学心理学教研室,西安710032 [3]第四军医大学人体解剖与组织胚胎学教研室,西安710032 [4]第四军医大学基础部教学实验中心,西安710032

出　　处：《神经解剖学杂志》2012年第4期397-400,共4页Chinese Journal of Neuroanatomy

摘　　要：目的:探讨创伤后应激障碍(PTSD)模型大鼠的焦虑抑郁行为改变以及乳头体神经元的活化情况。方法:采用单次延长应激(SPS)建立大鼠PTSD模型,采用自发活动、高架十字迷宫方法评价大鼠的自发活动能力和焦虑或抑郁行为;同时利用Fos蛋白免疫荧光染色方法观察乳头体神经元的活化情况。结果:SPS大鼠自发活动明显减少,焦虑样行为明显增多,乳头体内Fos免疫反应阳性神经元数量较正常大鼠显著增加。结论:SPS大鼠乳头体神经元显著激活,可能参与SPS所造成的PTSD反应。Objective： To investigate the behavioral change of anxiety and depression in the rats model of posttraumatic stress disorders （PTSD） and the neuronal activation in the mammillary body. Methods： By using single prolonged stress （SPS） to establish the PTSD animal model, open-field test （OF） and elevated plus maze （EPM） were used to evaluate rats＇ spontaneous acitivity and anxiety ＆ depression behavior, and the expression of c-Fos actived in the mammillary neu- ron after single prolonged stress were identified by immunofluorescence staing. Results： The spontaneous activity were significantly reduced, and the anxiolytic behavior were significantly increased, at the same time, the expression of Fos im- munoreactive neurons were increased significantly in the mammillary body in the SPS group compared to the control group. Conclusion： The neurons in the mammillary body in the SPS group were sigificantly activated, and it may be involved in the PTSD response caused by the SPS.

关 键 词：乳头体 FOS 免疫组织化学 高架十字迷宫 自发活动 

分 类 号：R749.5[医药卫生—神经病学与精神病学]