左炔诺孕酮下调survivin基因促进人子宫肌瘤细胞凋亡  

Levonorgestrel induces apoptosis through down-regulation of survivin gene expression in human uterine leiomyoma cells

作　　者：仇黎丽[1] 徐青[2] 徐昌芬[2] 

机构地区：[1]南京大学医学院附属鼓楼医院妇产科,江苏南京210008 [2]南京医科大学组织胚胎学系,江苏南京210029

出　　处：《基础医学与临床》2012年第8期926-929,共4页Basic and Clinical Medicine

基　　金：南京医科大学科技发展基金重点项目(2005NYDZD21);南京市医学科技发展项目(YKK06080)

摘　　要：目的探讨左炔诺孕酮(LNG)诱导人子宫肌瘤细胞(UtLMC)凋亡过程中survivin的表达变化。方法原代培养人子宫肌瘤细胞传代后,加入不同浓度LNG,以AO/EB双染法区分早、晚期凋亡细胞和坏死细胞;RT-PCR检测抑凋亡基因survivin mRNA的表达,Western blot测定survivin蛋白的表达。结果 10 mg/L LNG作用UtLMC后早期凋亡细胞多于阴性对照组,随着LNG剂量的增加,晚期凋亡细胞也逐渐增多,核浓聚、偏位,被染成桔红色;在10 mg/L以上LNG诱导的UtLMC凋亡中,survivin mRNA表达显著下降,蛋白表达由对照组的33.82±0.02下降至10.37±0.03(P<0.05)。结论一定浓度的左炔诺孕酮所诱导的人子宫肌瘤细胞凋亡,可能与抑制survivin抗凋亡活性相关。Objective To explore the function of Survivin in levonorgestrel （LNG）-induced apoptosis in cultured human uterine leiomyoma cells （UtLMC） in vitro. Methods AO/EB double staining was applied to discriminate the apoptotic cells from dead ones. The changes of survivin mRNA expressions in UtLMC after LNG treatment were detected by semi-quantitative reverse transcriptase polymerase chain reaction （RT-PCR） method. Furthermore, Western blot was used to determine survivin protein expression in LNG-indueed UtLMC apoptosis. Results The result of AO/EB double staining showed that there were more viable apoptotic cells in LNG-treated groups than control group and the number of non-viable apoptotie cells and dead cells increased while the concentration of LNG elevated. Survivin expression in LNG-treated cells was decreased both at mRNA and protein levels according to RT- PCR and Western blot results. Conclusions Levonorgestrel treatment may induce a substantial apoptotic response in UtLMC, which was enhanced with the raise of levonorgestrel concentration. It may be due to the marked down- regulation of survivin gene expression.

关 键 词：左炔诺孕酮 子宫肌瘤 细胞凋亡 SURVIVIN 

分 类 号：R737.33[医药卫生—肿瘤]