水通道蛋白3在非小细胞肺癌中的表达及与临床病理相关性的研究  

Correlation of Aquaporin 3 Expression with the Clinicopathologic Characteristics of Non-small Cell Lung Cancer

作　　者：李白翎[1] 金磊[1] 钟铿[1] 杜大海[2] 

机构地区：[1]第二军医大学附属长海医院胸心外科,上海200433 [2]第二军医大学附属长海医院实验诊断科,上海200433

出　　处：《中国肺癌杂志》2012年第7期404-408,共5页Chinese Journal of Lung Cancer

基　　金：上海市科学技术委员会医学引导基金资助项目(No.114119a9300)资助~~

摘　　要：背景与目的肺癌是严重威胁人类生存和发展的恶性疾病之一,本研究旨在探讨非小细胞肺癌(non-small cell lung cancer,NSCLC)组织中水通道蛋白-3(aquaporins3,AQP3)的表达,探讨其与NSCLC临床病理学之间的关系。方法应用免疫组织化学方法检测180例NSCLC组织中AQP3表达及微血管密度(micro vascular density,MVD)。结果 NSCLC组织中AQP3阴性表达率为13.9%(25/180),中等强度阳性表达率为37.2%(67/180),强阳性表达率为48.9%(88/180)。NSCLC组织中AQP3表达高于癌旁组织,有明显统计学差异(P<0.01)。AQP3高表达也同时伴随着MVD计数增高(P<0.01)。男性患者AQP3表达高于女性患者(P=0.003)。腺癌中AQP3的表达较鳞癌明显增强(P<0.001);有淋巴结转移的病例存在AQP3高表达(P=0.026)。NSCLC中AQP3的阳性表达率与肿瘤分化程度呈正相关,表现为AQP3的阳性表达率在高分化癌中明显高于低分化癌(P<0.001)。结论 AQP3在NSCLC的肿瘤血管生成和进展中起重要促进作用,AQP3可能为NSCLC治疗的新靶点。Background and objective Lung cancer is a major health problem worldwide. The aim of this study is to investigate aquaporin 3 （AQP3） expression and its relationship with the clinicopathologic characteristics of non-small cell lung cancer （NSCLC）. Methods AQP3 expression and the microvascular density （MVD） of tissue samples from 180 cases with NSCLC were detected by immunohistochemistry. Results AQP3 expression was negative in 25 （13.9%）, positive in 67 （37.2%）, and strongly positive in 88 （48.9%） of the 180 cases, which was significantly higher than that in the normal tissue （P〈0.01）. A significant correlation was found between AQP3 expression and MVD （P〈0.01）, whereas a high MVD was found among patients with strongly positive AQP3 expression. Male patients with positive or strongly positive AQP3 expression had significantly higher expression than female patients did （P=0.003）. AQP3 expression was more significantly enhanced in ad- enocarcinoma than that in squamous cell carcinoma （P〈0.001）. Statistical analysis indicated that the positive rate of AQP3 ex- pression in well-differentiated carcinoma was significantly higher than that in poorly differentiated tumors （P〈0.001）. Lymph node metastasis was positively correlated with high AQP3 expression （P=0.026）. Conclusion AQP3 expression was closely correlated with MVD in NSCLC, whereas high MVD was frequently found in tumors with high AQP3 expression. AQ.P3, as a therapeutic target for inhibiting high AQ.P3 expression in NSCLC tissues, may weaken cancer cell proliferation, invasion, and metastasis.

关 键 词：肺肿瘤 AQP3 微血管密度 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]