自体牙周膜细胞移植对狗牙周组织再生的影响  

Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs

作　　者：欧龙[1] 刘宏伟[1] 庞劲凡[1] 袁志萍[1] 马良[1] 

机构地区：[1]解放军总医院口腔科,北京100853

出　　处：《中华口腔医学杂志》2000年第1期44-46,I001,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 !(39770798);国家教委留学回国人员科研启动基金资助项目![外教司留(1997)436]

摘　　要：目的　对应用自体牙周细胞移植结合e PTFE膜引导的牙周组织再生的动物实验进行评价。方法　将 6只成年狗的 36颗牙分为实验组和对照组 ,每组 18颗牙。在人工制造的牙周缺损中 ,进行体外培养的自体牙周细胞移植结合GTR法为实验组 ,单纯应用GTR法为对照组。 6周后切片行牙周组织学观察。结果　实验组新生牙槽骨、牙周膜组织及牙骨质的修复再生效果明显好于对照组 (P <0 0 5 ) ;实验组牙槽骨再生高度平均为 (4 0 0± 0 13)mm ,对照组为 (3 0 9± 0 2 8)mm。结论应用自体牙周膜细胞移植结合eObjective To evaluate the effect of autogenous periodontal ligament cell (PDL) transplantation in periodontal tissue regeneration Methods PDL cells derived from the same dog were cultured with α MEM 1×10 5 cells of first passage were allowed to attach to the collagen membrane for 24 hours The membrane cells were transplanted into periodontal defect in the same dog Then the defects were covered with e PTFE membranes The defects covered only with e PTFE were the controls Eighteen teeth of 6 dogs for each group were included The dogs were sacrificed after 6 weeks Results The results showed that new bone formation (4 00±0 13)mm in test group was significantly higher than that of in control group (3 09±0 28)mm, P < 0 05 The new cementum formation in test group was better than control group ( P < 0 05) Conclusion The results suggested that PDL cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs

关 键 词：细胞移植 引导组织再生 牙周膜细胞 狗 

分 类 号：R780.2[医药卫生—口腔医学]