细胞凋亡在阿霉素肾病大鼠肾小球硬化过程中的意义  

Significance of apoptosis during glomerulosclerosis in adriamycin induced nephropathy in rats

作　　者：孙良忠[1] 岳智慧[1] 汤洁如[1] 陈述枚[1] 许韩师[1] 陶瑜[2] 关伟明[3] 

机构地区：[1]中山医科大学附属第一医院儿科,广州510080 [2]中山医科大学病理学教研室 [3]中山医科大学附属第一医院肾脏病实验室

出　　处：《中华儿科杂志》2000年第5期285-287,I005,共4页Chinese Journal of Pediatrics

摘　　要：目的　探讨细胞凋亡在肾小球硬化过程中的意义。方法　将 48只 8～ 10周雄性SD大鼠随机分为两组 ,于实验第 1天、第 2 1天对模型组分别从大鼠尾静脉注射 2mg/kg阿霉素 ,给对照组注射等量生理盐水。第 2次注药后 4、12、2 0、2 8周时分别处死每组各 4只大鼠 ,取肾组织行病理及电镜检测 ,并用末端标记法检测肾组织细胞凋亡情况。结果　模型组大鼠在 2 0和 2 8周时出现肾小球硬化 ,肾小管萎缩、间质单个核细胞浸润和纤维化 ;肾小球、肾小管凋亡细胞数显著增多 ,且肾小球硬化指数与肾小球、肾小管细胞凋亡指数显著相关 (rs 分别为 0 .831,0 .86 0 ,P均 <0 .0 1)。结论　大鼠阿霉素肾病肾小球硬化过程中 。Objective To determine the significance of glomerular and tubular cell apoptosis in glomerulosclerosis in rats induced by adriamycin. Methods Forty eight male SD rats were randomly divided into two groups: model group and control one. The 24 rats in model group were given adriamycin(doxorubicin)2 mg/kg intravenously on the 1st day and the 21st day, respectively. At the same time, normal saline solution was given to the other 24 rats in control group by the same way. Four rats in each group were sacrificed every 8 weeks from the 4th week after the second injection. The kidneys were studied by light microscopy and electronic microscopy. Apoptotic cells in the kidney were analyzed by in situ terminal deoxynucleotidyl transferase (TdT) mediated nick end labeling. Results In rats of model group, glomerulosclerosis, tubular atrophy and interstitial mononuclear cells infiltration and fibrosis emerged, and apoptotic cells in glomeruli and tubuli significantly increased at the 20th week and the 28th week. The glomerulosclerosis index was significantly correlated to the apoptotic indexes of glomeruli and tubuli ( r s =0.831, 0.860 respectively; P <0.01 for both). Conclusion Cells elimination in glomeruli and tubuli during glomerulosclerosis in adriamycin induced nephropathy was related to apoptosis.

关 键 词：肾小球硬化 阿霉素 细胞凋亡 大鼠 

分 类 号：R363[医药卫生—病理学]