多发性硬化患者外周血CD8^+记忆性T细胞亚群效应细胞因子的表达及其与病情严重程度的相关性  

Expressions of cytotoxity-related cytokines in CD8^+ memory T cell subsets in patients with multiple sclerosis and correlation between the cytokine levels and disease severity

作　　者：马文娟[1] 安春华[2] 

机构地区：[1]内蒙古医学院附属医院神经内科,内蒙古呼和浩特010050 [2]北京朝阳医院京西院区神经内科,北京100043

出　　处：《中国现代医学杂志》2012年第16期55-57,共3页China Journal of Modern Medicine

摘　　要：目的探讨多发性硬化患者外周血CD8+记忆性T细胞亚群效应细胞因子穿孔素和颗粒酶-B的表达及其与病情严重程度的相关性。方法采用扩展的残疾状况量表(EDSS)对19例多发性硬化患者的病情进行评估,采用流式细胞仪和直接免疫荧光法检测其CD8+记忆性T细胞亚群的数量,同时选择20例健康体检者作为对照。结果多发性硬化患者组CD8+效应记忆性T细胞和终末效应记忆性T细胞的数量均明显少于健康对照组,差异有显著性(P<0.05)。CD8+效应记忆性T细胞的数量与病情的严重程度呈负相关。结论 CD8+效应记忆性T细胞和终末效应记忆性T细胞的数量可作为一项评估多发性硬化病情严重程度的指标。[ Objective ] To investigate the expressions of perforin and granzyme-B in CD8＋ memory T cell subsets of peripheral blood in patients with multiple sclerosis and correlation between the cytokine levels and disease sever- ity. [ Methods ] The disease severity of 19 patients with untreated multiple sclerosis was reviewed using the extend- ed disability status scale （EDSS）. Flow cytometry and direct immunofluorescence assay were employed to detect the number of CD8＋ memory T cell subsets which expressed perforin and granzyme-B in peripheral blood of patients with multiple sclerosis and healthy controls. [ Results ] The number of effector memory CD8＋ T cells and terminal effector memory CD8＋ T cells in multiple sclerosis patients group were lower than that in healthy control group, with statistically significant difference（P 〈0.05）. The number of effector memory CD8＋ T cells was negatively correlated to disease severity of multiple sclerosis. [Conclusion] Effector memory CD8＋ T cells and terminal effector memory CD8＋ T cells can be used as an assessment indicator of the severity of multiple sclerosis.

关 键 词：多发性硬化 CD8+记忆性T细胞 穿孔素 颗粒酶-B 

分 类 号：R744.5[医药卫生—神经病学与精神病学]