源于栽培种花生的EST-SSR引物对野生花生扩增的多态性  

Genetic Diversity of Arachis Accessions Detected by EST-SSR from Cultivated Peanut(Arachis hypogaea L.)

作　　者：唐梅[1,2] 陈玉宁[2] 任小平[2] 黄莉[2] 周小静[2] 严海燕[1] 姜慧芳[2] 

机构地区：[1]中南民族大学生命科学学院,湖北武汉430074 [2]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《作物学报》2012年第7期1221-1231,共11页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB109300);国家自然科学基金项目(31000724);农作物种质资源保护项目(NB2010-2130135-28B);国家现代农业产业技术体系建设项目(CARS-14-种质资源评价)资助

摘　　要：以花生属86份野生近缘种和3份栽培种为材料,利用从栽培种花生中开发设计的EST-SSR引物,分析其对野生花生扩增的有效性,探讨EST-SSR引物用于花生资源遗传多样性研究的适用性。随机选取235对EST-SSR引物进行筛选,223对EST-SSR引物均扩增出条带,有效性为94.89%,其中能检测到多态性的53对引物在89份资源中共扩增出238条带,包括206条多态性带。每对引物能扩增出1～12多态性带,平均3.89条,多态性指数为0.044～4.040,平均1.173。统计分析结果表明,89份花生种质材料间的平均相似系数为0.685,变异范围为0.442～0.976,在遗传距离为0.408处,分成2大组9小组,栽培种花生被聚在花生区组中,相同区组的材料基本被聚在一起,A.duranensis与栽培种花生(A.hypogaea L.)的关系较近,聚类结果与花生属的植物学分类基本一致。对含油量和基因型数据相关性分析和t检验发现,POCR437-180/170等6对标记可以作为含油量相关分析标记筛选的后备标记。用这6对标记的引物序列搜索cDNA文库和BLAST库,发现引物POCR437序列对应丙二酸单酰-CoA-ACP转酰酶和酰基载体蛋白的编码基因,这2种蛋白质参与脂肪酸的合成。The amplification efficiency and application potential for the research on peanut genetic diversity by using culti- vars-derived EST-SSR primers in 89 Arachis aeecssions were assessed. Based on randomly selected 235 EST-SSR primer pairs, we detected 223 amplification bands in the 89 accessions with the amplification efficiency of 94.89%. Among them, 53 primer pairs exhibited 206 polymorphic bands, which showed 1-12 bands per primer pair with an average of 3.89. The polymorphism index ranged from 0.044 to 4.040 with an average of 1.173. The average similarity coefficient was 0.685 among 89 peanut acces- sions, ranging from 0.442 to 0.976. The accessions from the same section were mainly clustered together, peanut cultivars were grouped into the section of Arachis, and A. duranensis had a close relation to cultivated peanut, which is coincident with botanical classification. According to the statistical analysis from the results of oil content and SSR data in wild peanuts, some interesting bands such as POCR437-180/170 were found to be potential for candidate markers related to oil content. BLAST analysis indi- cated that the alignment sequence of primer POCR437 corresponded to the coding genes of malonyl-CoA-ACP transacylase and acyl-carrier-protein, both of which are involved in fatty acid synthesis.

关 键 词：野生花生 EST-SSR 有效性 聚类分析 

分 类 号：S565.2[农业科学—作物学]