水牛DGAT1基因第8外显子群体遗传特征分析  

Genetic Characteristics of Exon 8 of DGAT1 Gene in Buffalo(Bubalus bubalus)

作　　者：熊飞[1] 李卫真[1] 刘丽仙[2] 袁峰[1] 李大林 苗永旺[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南农业职业技术学院畜牧兽医系,云南昆明650212 [3]云南省家畜改良工作站,云南昆明650225 [4]云南巴福乐水牛技术研究所,云南昆明650021

出　　处：《西南农业学报》2012年第3期1101-1105,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省应用基础研究重点项目(2007C0003Z);国家自然科学基金项目(30660024);云南省应用基础研究计划面上项目(2006C0034M;2007C057M;2010ZC243);国家高技术研究发展计划(863计划)项目(批准号:2008AA101001);云南省教育厅科学研究基金项目(2010Y344;09C0197);云南省财政厅基金项目(槟榔江水牛种质特性研究)

摘　　要：为了阐明河流型和沼泽型水牛DGAT1基因的第8外显子的群体遗传差异和特征,采用PCR-SSCP和PCR产物直接测序技术对11个水牛群体402份样品进行了群体变异检测,并结合GenBank数据库上已发表的牛科物种DGAT1基因序列进行了比较分析。结果显示,河流型和沼泽型水牛DGAT1基因第8外显子序列一致,序列全长为75 bp;与普通奶牛序列相比,河流型和沼泽型水牛DGAT1基因第8外显子都为K等位基因,揭示其与水牛的高乳脂率、低泌乳量有关。序列比对发现K等位基因在牛科物种中普遍存在。本研究结果揭示了水牛中DGAT1基因第8外显子的遗传特征,可为其奶用性状选育利用提供遗传背景资料。In order to make clear the genetic differences and population characteristics about exon 8 of DGAT1 gene, genetic variation of 402 samples from I 1 buffalo pepulations were assayed by employing PCR-SSCP and PCB direct sequencing method. The sequences of DGATI of the bovine species in GenBank database were pooled together, the comparative genomios analysis was conducted. The rcsults showed that the sequences of DGAT] exon8 of river buffalo were identical with swamp buffalo, their length was 75 bp. Compared with the sequence of Hol- steins cow, the sequences ofDGAT1 exon8 in swamp and river buffalo were a]]e]e K, which indicate that it was associated with high milk fat percentage and low milk yield in buffalo. After sequence alignment, were allele K was universal existence in bovine species. The genetic characteristics of DGAT1 exon 8 disclosed in this study could provide genetic background information for the selection and utilization of milk traits in buffalo breeding.

关 键 词：水牛 DGAT1基因 PCR-SSCP K232A变异 

分 类 号：S823[农业科学—畜牧学]