VEGF-C和VEGFR3在细支气管肺泡癌组织中的表达及意义  

Expression and significance of VEGF-C and VEGFR3 in bronchioalveolar carcinoma

作　　者：邹亮[1] 高慧淳[1] 欧阳莉[1] 朱敏媛[1] 刘文[1] 周建洪[1] 

机构地区：[1]南昌大学第三附属医院,南昌330006

出　　处：《山东医药》2012年第27期23-25,共3页Shandong Medical Journal

基　　金：江西省科技厅支撑计划项目(项目编号:2009BSB11108)

摘　　要：目的探讨血管内皮生长因子(VEGF)-C及其受体(VEGFR3)在细支气管肺泡癌(BAC)发生、发展中的作用。方法选择60例BAC患者的手术切除癌组织(BAC组)及其癌旁肺组织10例(对照组),采用免疫组化Elivison法检测VEGF-C和VEGFR3表达,并用D2-40标记淋巴管,计算微淋巴管密度(LVD);分析各指标间的关系及与肿瘤临床病理参数的关系。结果 BAC组VEGF-C、VEGFR3阳性率及LVD均显著高于对照组(P均<0.01);VEGF-C在BAC组非黏液型阳性率显高著于黏液型(P<0.01)、伴淋巴结转移组阳性率明显高于无转移组(P<0.05),VEGFR3在BAC组伴淋巴结转移者阳性率明显高于无转移者(P<0.05),LVD在BAC组伴淋巴结转移者阳性率明显高于无转移者(P<0.05);BAC组VEGF-C、VEGFR3与LVD之间均呈显著正相关(P<0.05)。结论VEGF-C与VEGFR3在BAC新生淋巴管形成和淋巴结转移中起协同作用,阻断其信号转导通路有望成为抗BAC淋巴结转移治疗的新靶点。Objective To investigate the roles of vascular endothelial growth factor（ VEGF）-C and its receptor VEG-FR3 in the formation and progression of bronchioalveolar carcinoma（BAC）. Methods Sixty cases of BAC excissed from patients were enrolled in BAC group, control group were 10 cases of lung tissue adjacent to BAC. Expression of VEGF-C and VEGFR3 were detected by using immunohistochemical. Lymphatic vessels were identified by D2-40 to caculate lym- phaticmicrovessel density（LVD）. Relationship between each index and their relationships with clinicopathologic parameters were analyzed. Results Positive rate of VEGF-C, VEGFR3 and LVD in BAC group was all notably higher than those in control group （P 〈 O. O1 ） ; postive rate of VEGF-C in nonmucinous cases was notably higher than that in mucinous cases （ P 〈 0. O1 ）, postive rate of VEGF-C in cases with node metastasis was obviously higher than that in cases without node me-tastases （ P 〈 0.05 ）, positve rate of VEGFR3 in node metastasis was obviously higher than that in cases without node metas-tases（ P 〈 0. 05）. LVD in node metastasis was obviously higher than that in cases without node metastases （P 〈 0.05 ）; there was a positive correlation among VEGF-C, VEGFR3 and LVD （P 〈 0.05 ）. Conclusion There is a synergistic effect between VEGF-C and VEGFR3 in promoting lymphangiogenesis and lymph node metastasis in BAC. By blocking the signal transduction pathway is expected to be a new target of anti-lymph node metastasis in BAC.

关 键 词：细支气管肺泡癌 血管内皮生长因子-C 血管内皮生长因子受体 微淋巴管密度 淋巴结转移 

分 类 号：R734.1[医药卫生—肿瘤]