Ce(Ⅲ)-硝基磺酚C-人血清白蛋白-BRIJ-35体系的光谱性质及其分析应用  

Study on the Absorption Spectrum of Ce(Ⅲ)-Nitrosulfonphenol C-HSA-BRIJ-35 System and its Analytical Application

作　　者：冯爱青[1] 胡秋娈[2] 孙津鸽[2] 刘尚才[3] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]洛阳师范学院化学化工学院,河南洛阳471022 [3]洛阳正骨医院,河南洛阳471002

出　　处：《洛阳师范学院学报》2012年第8期60-62,共3页Journal of Luoyang Normal University

基　　金：河南省科技厅科技攻关重点项目(102102110154);河南省教育厅自然科学研究计划项目(2011A150020)

摘　　要：Ce(Ⅲ)-硝基磺酚C与无色的蛋白质在pH=3.48的HAc-NaAc缓冲介质中生成可溶性蓝色缔合物,λmax=700 nm,比试剂本身的最大吸收波长560 nm红移了140 nm,ε=3.95×105L·mol-1·cm-1,蛋白质在20～140ug/mL(HSA)范围内遵循比尔定律.探讨了反应的最佳条件及初步反应机理.方法灵敏度高,重现性好,操作简便,基本无干扰.BRIJ-35存在,灵敏度提高19%.用于血清中蛋白质的测定,和目前临床检验中使用的双缩脲法相比,灵敏度提高了11倍.Ce （Ⅲ ） -Nitrosulfophenol C could combine with protein form blue complex in HAc-NaAc buffer at pH 3.48, which presents a maximum absorption at 700nm with 140 nm of red shift compared to Nitrosulfophenol C itself. Its apparent molar absorbency index and linear range were 3.95 × 105 L · mol-1· cm-1 and 20 - 140 μg/mL. The optimum conditions and the initial reaction mechanism were studied. The method is simple, fast, high sensitivity and good reproducibility. Sensitivity increased 19% with the presence of BRIJ-35. The sensitivity of the method is the Biuret method 11 times. The method has been used for the determination of Human serum protein with satisfactory results.

关 键 词：人血清白蛋白 Ce(Ⅲ)-硝基磺酚C 分光光度法 

分 类 号：O657.3[理学—分析化学]