红芪多糖联合X线对HepG-2细胞DNA损伤的影响  

DNA damage in HepG-2 cells induced by Hedysarum polybotys saccharides and X-ray irradiation

作　　者：董玉梅[1] 王小虎[1,2] 寇炜[3] 刘凯[1] 孙少伯[1] 李应东[1] 

机构地区：[1]甘肃中医学院中西医结合系,甘肃兰州730000 [2]甘肃肿瘤医院放疗科,甘肃兰州730050 [3]兰州大学基础医学院,甘肃兰州730020

出　　处：《实用肿瘤杂志》2012年第4期344-349,共6页Journal of Practical Oncology

基　　金：国家自然科学基金(81160478);甘肃省重离子等射线治疗肿瘤研究科技创新团队资金(098TTCA009);甘肃省2009年科技重大专项(092NKDA017)

摘　　要：目的研究红芪多糖联合X线对人肝癌体外培养的HepG-2细胞DNA损伤的影响,并初步探讨其抑制肿瘤细胞增殖的机制。方法 体外培养HepG-2细胞,加不同浓度的红芪多糖处理12、24、36、48小时后,CCK-8法测定细胞的生长抑制率;用单细胞凝胶电泳技术观察红芪多糖联合直线加速器发射的6MV X线辐照HepG-2细胞后,其DNA的损伤情况。结果 HPS(5～100 mg/L)可抑制HepG-2细胞的增殖,具有时间和浓度依赖性;单细胞凝胶电泳法显示浓度为25 mg/L HPS作用12小时后可见明显的彗星状拖尾,而对照组细胞呈明显的圆形;HPS作用12小时后,进行2 Gy的X线照射后可见更加明显的彗星状拖尾,而对照组细胞呈明显的圆形;而2 Gy的X线照射后,再给予浓度为25 mg/L的HPS作用,亦见更加明显的彗星状拖尾,而对照组细胞呈明显的圆形;经HPS联合X线处理后的HepG-2细胞,其DNA损伤程度与单用HPS处理的细胞的DNA损伤程度相当,但优于单用X线处理;X线辐照后,立即给予HPS处理,HepG-2细胞的DNA损伤程度优于单用X线、单用HPS及HPS联合X线处理。结论 HPS可抑制HepG-2细胞的增殖;HPS和(或)X线可对HepG-2细胞的DNA造成显著的损伤;HPS可增敏X线损伤HepG-2细胞;HPS抑制HepG-2细胞损伤的DNA双链的修复可能是其增敏X线治疗肿瘤的机制之一。Objective To investigate the effect of Hedysarum polybotys saccharides （ HPS ） combined with X ray on human hepatocellular carcinoma HepG-2 cells in vitro. Methods The cultured HepG-2 cells were treated with different concentrations of HPS and/or exposed to 2 Gy X-ray irradiation. The proliferation of HepG-2 cells was examined by CCK-8 assay after 12,24,36,48 h, respectively; the DNA damage of HepG-2 cells was studied by single cell gel electrophoresis（SCGE）. Results The proliferation of HepG-2 cells was inhibited after HPS（5 ~ 100 mg/L） treatment in vitro in a dose- and time-dependent manner. SCGE showed comet- like tails, when HepG-2 cells were treated with X-ray and/or HPS. The severity of DNA damage in HepG-2 cells treated with HPS and X-ray was similar to that observed in HepG-2 cells treated with HPS alone, but was more severe than that with X-ray alone. When HPS was added to cells immediately after X-ray irradiation, the DNA damage was more severe than that in cells treated with X ray, HPS or HPS combined with X-ray. Conclusions HPS can inhibit the proliferation of HepG-2 cells and HPS treatment alone and combined with X ray exposure can both damage DNA of HepG-2 cells remarkably ,which may he associated with the radiosensitizing effect of HPS.

关 键 词：肝肿瘤/遗传学 DNA损伤/辐射效应 彗星试验 X线/副作用 多糖类/药理学 红芪/药理学 

分 类 号：R735.7[医药卫生—肿瘤] Q3[医药卫生—临床医学]