大肠癌组织库建立及质量控制的经验  

Establishment of colorectal cancer tissue bank and quality control

作　　者：王聪[1] 张大庆[1] 赵盈盈[1] 仇长青[1] 杜勇[2] 李海[3] 翟惠虹[4] 

机构地区：[1]宁夏医科大学临床医学院,宁夏银川750004 [2]宁夏医科大学附属总医院外科实验室,宁夏银川750004 [3]宁夏医科大学附属总医院结直肠外科,宁夏银川750004 [4]宁夏医科大学附属总医院消化内科,宁夏银川750004

出　　处：《实用肿瘤杂志》2012年第4期406-410,共5页Journal of Practical Oncology

基　　金：宁夏高等学校科学技术研究项目

摘　　要：目的建立规范的大肠癌组织库,收集高质量的标本应用于肿瘤研究。方法 筹备建立组织库所需资金并通过伦理委员会同意,建立标本收集、储存的标准操作流程;收集临床患者的术后病灶标本并冷冻储存;对组织库及标本进行严格的质量控制。结果 本研究建立了标准的组织库操作流程;建库1年收集大肠癌患者组织标本587个、血浆标本549个;随机选取样本的质量检测显示样本DNA、RNA为高质量,癌组织中肿瘤细胞比例>80.0%;标本临床资料完整。结论 质量控制显示收集的冰冻大肠癌组织其分子质量可靠,并有完整的病例临床资料,可充分满足研究的需要。Objective To establish a standardized colorectal cancer tissue bank. Methods The project and protocols were approved by Ethical Committee of the hospital. Colorectal cancer tissues were collected from patients and stored in freezer. Results A standard protocol for sample collection was developed. During one year period 587 samples of eoloreetal cancer tissue and 549 serum samples were stored. RNA and the genomic DNA of the randomly selected specimens were of good quality. The percentages of cancer cells in cancer samples were greater than 80% and the specimens collected were associated with complete clinical data. Conclusion The eoloreetal cancer tissue bank established in this study can fully meet the requirements of clinical and basic research.

关 键 词：结直肠肿瘤/病理学 组织库 生物标本库/标准 质量控制 

分 类 号：R735.34[医药卫生—肿瘤]