N-甲基-D-天冬氨酸受体1在一过性肠道感染大鼠内脏高敏感形成中的意义  

Role of N-methyl-D-aspartate receptor one in visceral hypersensitivity forming of transient intestinalinfection induced rat

作　　者：杨小军[1] 沈蕾[1] 钱伟[1] 官阳[2] 侯晓华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化科,武汉430022 [2]华中科技大学同济医学院病理学系,超微病理研究室

出　　处：《中华消化杂志》2012年第7期455-458,共4页Chinese Journal of Digestion

基　　金：国家自然科学基金（30670957）

摘　　要：目的研究N-甲基-D-天冬氨酸受体1（NMDA-R1）在SD大鼠一过性肠道感染旋毛虫后形成的内脏高敏感中的作用。方法40只SD大鼠均分为健康对照组、内脏高敏感组、0．9％氯化钠溶液组、MK-801组，其中内脏高敏感组、0．9％氯化钠溶液组和MK-801组分别予旋毛虫感染造模。8周后，0．9％氯化钠溶液组和MK-801组分别予0．9％氯化钠溶液和NMDA-R1拮抗剂MK-801。观察各组在20、40、60和80mmHg（1mmHg-0．133kPa）结直肠扩张压力下腹壁肌电活动反应。测定腹壁肌电活动后，取大鼠结肠标本，采用Western印迹检测NMDA-R1蛋白水平的表达。统计学处理采用Bivariate相关分析和LSD检验。结果健康对照组、内脏高敏感组、0．9％氯化钠溶液组和MK-801组腹壁肌电图曲线下面积（AUC）与扩张压力之间均有相关性（r=0．823、0．618、0．913、0．889，均P〈0．01）。在20、40、60和80mmHg扩张压力下，MK-801组与内脏高敏感组AUC间差异均有统计学意义（LSD检验，P值均〈0．05），MK-801组AUC均小于健康对照组，但只在80mmHg扩张压力下差异有统计学意义（LSD检验，P=0．029）。MK-801组NMDA-R1蛋白积分吸光度相对值为1．238±0．210，较内脏高敏感组（2．231±0．450）和0．9％氯化钠溶液组（2．104±0．220）均显著降低（LSD检验，P=0．025和0．046），内脏高敏感组NMDA-R1蛋白积分吸光度相对值和0．9％氯化钠溶液组较健康对照组显著升高（LSD检验，P=0．007和0．014）。结论内脏高敏感的形成与突触后NMDA-R1的高表达密切相关，抑制肠道神经系统中NMDA-R1的表达可调节内脏高敏感性。Objective To investigate the role of N-methyl-D-aspartate receptor one（NMDA-R1） in visceral hypersensitivity forming of Sprague-Dawley rat model induced by transient Trichinella spiralis intestinal infection. Methods Forty male Sprague-Dawley rats were divided into healthy control group, visceral hypersensitivity group, 0. 9% NaC1 group and MK-801 group. The rats in visceral hypersensitivity group, 0.9 %NaCI group and MK-801 group were given Trichinella spiralis to establish infection model. After eight weeks, the rats in 0.9%NaC1 group and MK-801 group were given 0.9%NaCl and MK-801 respectively. The abdominal muscular electrical activity of each group was observed under 20, 40, 60 and 80 mm Hg （1 mm Hg=0. 133 kPa） colorectal distension pressure. After abdominal muscular electrical activity recorded, rats were sacrificed and the colon specimenswere collected. The expression of NMDA-R1 at protein level was detected by Western blot. The data were analyzed by LSD test and Bivariate correlation. Results There was significant correlation between areas under curve （AUC） of abdominal muscular electrical activity electromyography and distension pressure in healthy control group, visceral hypersensitivity group, 0. 9% NaC1 group and MK-801 group （r=0.823, 0.618, 0.913, 0.889 respectively, all P〈0. 01）. Under 20, 40, 60, 80 mm Hg distension pressure, there was significant difference in AUC between MK-801 group and visceral hypersensitivity group （LSD test, all P%0.05）. The AUC of MK-801 group was lower than that of healthy control group, but there was significant difference （LSD test, P = 0. 029 ） only when distension pressure was at 80 mm Hg. The integral optical density value of NMDA-R1 protein of MK- 801 group （1. 238 ± 0. 210） was significantly lower than that of visceral hypersensitivity group （2. 231±0. 450） and 0.9MNaC1 group （2. 104±0. 220） （LSD test, P=0. 025,0. 046）. The IA value of NMDA-R1 protein of visceral hypersensitivity group and 0.9 %NaC1 group was significant

关 键 词：受体 N-甲基-D-天冬氨酸 内脏 肠炎 肠易激综合征 

分 类 号：R363[医药卫生—病理学]