结肠癌中S100A2的表达及其与Wnt/β-catenin通路的相关性  

Correlation of S100A2 expression and wnt/β-catenin signaling pathway in human colon cancer

作　　者：杨勇[1] 朱明才[1] 段亮[2] 周兰[2] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆400016 [2]重庆医科大学医学检验系临床检验诊断学教育部重点实验室

出　　处：《第三军医大学学报》2012年第15期1576-1579,共4页Journal of Third Military Medical University

摘　　要：目的探讨钙结合蛋白S100A2与Wnt/β-catenin信号通路关键分子β-catenin、pGSK-3β在人结肠癌组织中的表达和意义以及相关性。方法采用免疫组化和Western blot方法检测42例结肠癌及癌旁正常组织中S100A2、β-catenin和pGSK-3β的表达情况,分析它们与结肠癌临床病理参数的关系,进一步采用Pearson等级相关分析其相关性。结果①免疫组化显示S100A2和β-catenin主要定位于癌细胞核和(或)细胞质,而pGSK-3β主要定位于癌细胞质;②Western blot结果显示S100A2、β-catenin和pGSK-3β在结肠癌组织中均高表达,明显高于癌旁正常组织(P<0.01),三者在结肠癌DukesC+D期中的表达明显高于A+B期(P<0.01),在有淋巴结转移组的表达明显高于无淋巴结转移组(P<0.01);③S100A2分别与β-catenin、pGSK-3β的表达呈明显正相关(r=0.637,P<0.01;r=0.626,P<0.01)。结论 S100A2可能通过调节Wnt/β-catenin通路关键分子而参与结肠癌的发生、发展。Objective To investigate the correlation of the expression of S100A2 with 2 key molecules of wnt/β-catenin signaling pathway,β-catenin and pGSK-3β in human colon cancer and their clinical significance.Methods Expression of S100A2,β-catenin and pGSK-3β in 42 colon cancer tissue samples was detected by immunohistochemical assay and Western blotting.Their relation with the clinicopathological characteristics of colon cancer was studied,and their correlation was analyzed by Pearson rank analysis.Results Immunohistochemistry demonstrated that S100A2 and β-catenin were mainly located in the cancer cells nuclear and/or cytoplasm,while pGSK-3β was located in the cytoplasm.Western blotting showed that the expression of S100A2,β-catenin and pGSK-3β was significantly higher in tumor tissue than normal tissue（P〈0.01）.Meanwhile,the expression was markedly higher in Dukes stage C＋D than in Dukes A＋B（P〈0.01）,in the cases with lymph node metastasis than those without metastasis（P〈0.01）.The expression of S100A2 was positively related with that of β-catenin and GSK-3β,respectively（r=0.637,P〈0.01;r=0.626,P〈0.01）.Conclusion S100A2 may participate in the incidence and development of colon cancer via adjusting the key molecules of Wnt/ β-catenin pathway.

关 键 词：结肠癌 S100A2 WNT/Β-CATENIN 

分 类 号：R735.35[医药卫生—肿瘤] R730.23[医药卫生—临床医学]