周期性张应力作用下成骨样细胞MG-63中c-fos基因和丝状肌动蛋白相互关系的研究  

The relationship between c-fos gene and filamentous actin cytoskeleton in MG-63 osteoblasts under cyclic tensile stress

作　　者：堵安庆[1,2] 王羽[3,4] 赵森[1,2] 李炜鹏[1,2] 赵志河[1,2] 

机构地区：[1]四川大学华西口腔医院正畸科,成都610041 [2]口腔疾病研究国家重点实验室,四川大学,成都610041 [3]中山大学光华口腔医学院·附属口腔医院正畸科,广州510055 [4]广东省口腔医学重,点实验室,广州510055

出　　处：《华西口腔医学杂志》2012年第4期430-433,438,共5页West China Journal of Stomatology

摘　　要：目的探讨成骨样细胞MG-63中c-fos基因和细胞骨架丝状肌动蛋白(F-actin)的相互关系。方法对MG-63细胞施加周期性张应力,频率为0.5 Hz,细胞应变为2 000μstrain,按3、6、12 h不同时间段进行加载,采用荧光定量聚合酶链反应(PCR)检测c-fos mRNA的变化,筛选最佳加载时间点,并作为实验组和0 h组进行对比,检测在细胞松弛素D作用下c-fos mRNA和F-actin的表达变化。结果周期性张应力可以诱导c-fos mRNA的表达增加,至3 h达到峰值;在周期性张应力作用下,MG-63细胞中F-actin的结构、排列发生改变,但荧光强度无明显变化;经细胞松弛素D处理后,张应力作用下的MG-63细胞的应力纤维明显减少,F-actin荧光强度降低,c-fos mRNA表达受抑制。结论周期性张应力作用下,F-actin的量并没有明显变化,只是出现了结构上的重组,且重组的是既有的F-actin。F-actin重组是应力诱导c-fos基因高表达的一个重要环节。Objective To investigate the relationship between c-fos gene and filamentous actin（F-actin） in MG63 osteoblasts under cyclic tensile stress.Methods MG-63 osteoblasts were subjected to cyclic tensile stress（0.5 Hz, 2 000 μstrain） for 3,6,and 12 h.The changes of c-fos gene were investigated by fluorescent quantitation polymerase chain reaction.Then the best loading time group was screened as the experimental group compared with 0 h group.The changes of F-actin and c-fos were investigated with or without cytochalasin D treatment.Results Cyclic tensile stress induced high expression of c-fos mRNA,and peaked at 3 h.After loading,F-actin had a structure reorgani-zation,but had no change in expression.After cytochalasin D treatment,the formation of stress fibers and the fluo-rescence intensity of F-actin cytoskeleton significantly reduced,meanwhile the c-fos mRNA expression was inhibited.Conclusion After loading,there is only structure reorganization for F-actin,and the expression has not any change.That means the remodeling F-actin is the existing one.F-actin reorganization is an important part in c-fos gene ex-pression induced by stress.

关 键 词：周期性张应力 成骨细胞 C-FOS基因 丝状肌动蛋白 

分 类 号：R780.2[医药卫生—口腔医学]