肾透明细胞癌中氧化应激蛋白的表达  

Expression of oxidative stress proteins in the clear-cell renal cell carcinoma

作　　者：田美娟[1] 李雨遥[1] 罗长琴[1] 吕美玲[1] 杨谨[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤内科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2012年第8期851-855,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81172171)

摘　　要：目的:探讨人肾透明细胞癌细胞株RLC-310与人正常肾近曲小管上皮细胞株HK-2,肾癌及其癌旁组织中氧化应激蛋白表达的差异。方法:体外培养RLC-310和HK-2,采用PF-2D蛋白分级分离系统分离细胞总蛋白,选取差异蛋白组分进行毛细管LC-ESI-MS/MS分析,结合蛋白质数据库检索对差异蛋白进行鉴定,并对代表性氧化应激差异蛋白采用免疫组织化学方法进行验证。结果:两个细胞株经串联质谱分析共鉴定出12个氧化应激差异表达蛋白,分别是peroxiredox-in-1(PRX-1)、peroxiredoxin-6(PRX-6)、superoxide dismutase[Cu-Zn]SOD1、glutathione peroxidase 1、catalase、glutathionesynthetase、glutathione S-transferase Pi(GSTPi)、thioredoxin、热休克蛋白10(heat shock protein,HSP10)、HSP 60、HSP 70和HSP 90。其中PRX-6、HSP 60、GSTPi三种代表性差异蛋白在人肾透明细胞癌细胞株RLC-310和人正常肾近曲小管上皮细胞株HK-2中均有表达,前者的表达水平较后者显著升高(P<0.05),这三种蛋白在肾癌组织中表达也较癌旁组织显著升高(P<0.05)。结论:人肾透明细胞癌中存在抗氧化应激蛋白的差异表达,这些氧化应激蛋白的异常表达在阻止肾癌细胞氧化损伤中起一定作用。AIM： To investigate the expression of the oxidative stress proteins in human clear-cell renal cell carcinoma（CRCC） cell line（RLC-310） and normal renal proximal tubule epithelial cell line（HK-2）,the CRCC and the corresponding normal renal tissues.METHODS： RLC-310 and HK-2 cells were cultured in vitro.Total proteins of the two cell lines were separated by PF-2D protein fractionation system.The differentially expressed proteins of the two cell lines were analyzed using capillary LC-ESI-MS/MS and identified using the protein database.The representative differential oxidative stress proteins were verified by immunohistochemistry in the CRCC and corresponding normal renal tissues.RESULTS： Twelve differentially expressed oxidative stress proteins were identified,including peroxiredoxin-1,peroxiredoxin-6（PRX-6）,superoxide dismutase SOD1,catalase,glutathione peroxidase 1,glutathione synthetase,glutathione S-transferase Pi（GSTPi）,thioredoxin,heat shock protein 10（HSP10）,HSP60,HSP70 and HSP90.Three representative differential proteins PRX-6,HSP60 and GSTPi were both expressed in RLC-310 and HK-2,and the levels of these proteins were significantly higher in RLC-310 than those in HK-2（P0.05）.The levels of these proteins were significantly higher in CRCC than those in corresponding normal renal tissues（P0.05）.CONCLUSION： A series of oxidative stress proteins are overexpressed in the CRCC.They play an important role in preventing oxidative damage of CRCC cells.

关 键 词：氧化应激蛋白 透明细胞癌 肾癌 

分 类 号：R737.11[医药卫生—肿瘤]