去势大鼠血管内皮结构损伤的研究  

Study of vascular endothelial injury in male castrated rats

作　　者：匡琳[1] 李艳香[2] 余娇[2] 张丽[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院妇产科,杭州310001 [2]上海交通大学医学院附属第九人民医院内分泌代谢科

出　　处：《中华医学杂志》2012年第27期1929-1931,共3页National Medical Journal of China

摘　　要：目的探讨雄性大鼠去势后血管内皮的损伤。方法8周龄sD雄性大鼠30只随机分为3组：对照组（假去势）8只；去势组11只；去势后雄性激素替代治疗组11只。10周后，取主动脉血管，观察血管形态学变化和内皮细胞超微结构改变；用TUNEL法检测血管内皮细胞凋亡情况；RT-PCR检测bcl-2mRNA表达情况。结果与正常对照组相比去势组主动脉内皮结构排列紊乱，内皮细胞结构不完整，有脱落现象；替代组动脉内膜病变明显较去势组减轻。TUNEL检测结果示与对照组相比，去势后大鼠主动脉内皮细胞凋亡数量（61％±20％）较对照组（24％±16％）明显增多（P〈0．05）。bcl-2mRNA相对表达量在去势组（0．59±0．09）较对照组明显下调（P〈0．05），而替代组（0．74±0．24）与对照组间差异无统计学意义（P〉0．05）。结论雄性大鼠去势后，体内雄激素低于生理水平时，血管内皮结构损伤明显。Objective To explore the injury of vascular endothelium in male castrated rats. Methods A total of 30 eight-week-old adult male SD rats were randomly divided into 3 groups：control （ n = 8 ） ; castration （ n = 11 ） （ low androgen） and replacement （ n = 11 ） （ androgen dosing after castration）. After 10 weeks of treatment or castration, the rats were sacrificed to obtain aortic specimens. Arterial morphological changes were observed by hematoxylin and eosin staining and electron microscope. Endothelial cell chromatin degradation was detected by the assay of terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL）. The mRNA level of bcl-2 was analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. Results Compared with the control group, the arterial structure was disorderly and had shedding of endothelium in the castration group. More apoptotic endothelial cells were found in the castration group（61% ±20% ）than the other two groups （P 〈0. 05）. Compared with the control group, the bcl-2 mRNA level decreased significantly in the castration group （ P 〈 0.05 ） . However no significant difference existed in the replacement group （ P 〉 0. 05 ）. Conclusion Low androgen may damage the structure of vascular endothelium in male castrated rats.

关 键 词：雄激素 血管 内皮损伤 

分 类 号：R363[医药卫生—病理学]