姜黄素在治疗白血病过程中对心肌细胞氧化损伤的保护作用  

The effect of activation of Nrf2-ARE by curcumin to oxidative damage of myocardial cell by As_2O_3

作　　者：刘红艳[1,2,3] 郭静明 王海燕[1,2,3] 冉昌丽 唐芳蓉[1,2,3] 阎瑞[1,2,3] 

机构地区：[1]三峡大学第一临床医学院 [2]宜昌市中心人民医院血液科 [3]三峡大学肿瘤研究所,湖北宜昌443003

出　　处：《现代肿瘤医学》2012年第8期1685-1687,共3页Journal of Modern Oncology

摘　　要：目的:探讨姜黄素在治疗白血病的过程中,对心肌细胞氧化损伤的保护作用。方法:取原代培养大鼠心肌细胞,随机分为对照组、As2O3组和姜黄素组(激活组),MTT法检测各组心肌细胞存活力;Western blot法检测Nrf2和ARE的表达;分光光度法检测谷胱甘肽(glutathione,GSH)、超氧化物岐化酶(SOD)含量。结果:姜黄素组的心肌细胞氧化损伤的细胞存活力、细胞内GSH及SOD的水平较对照组显著提高(P<0.01)。结论:姜黄素可通过激活Nrf2-ARE通道,有效拮抗心肌细胞氧化损伤,为As2O3治疗各种类型白血病引起心肌毒性的预防和治疗提供新的靶点和思路。Objective：To observe the expression of GSH and SOD through activation of Nrf2 - ARE by curcumin and its effection to oxidative damage of myocardial eel1 by As2O3. Methods： The rat myocardial cell in primary culture were divided into control group, As203 group and activation of Nrf2 -ARE group. The viability of myocardial cell was tested by MTT, and the expression of Nrf2 and ARE by Western blot. The amount of GSH and SOD were tested by speetrophotometry. Results：It can improve the viability of myocardial cell by activiating Nrf2 -ARE （P 〈 0.01 ）. It can increase the amount of GSH and SOD（ P 〈 0.01 ）. Conclusion：The activation of Nrf2 -ARE can antagonize oxi- dative damage of myocardial cell availably. It provides a new idea of treatment to leukemia by As2O3.

关 键 词：姜黄素 三氧化二砷 Nrf2-ARE 血清谷光甘肽 超氧化物岐化酶 

分 类 号：R733.7[医药卫生—肿瘤]