C3aR和STAT3因子作为免疫复合物肾脏损伤生物标记物的研究  

Biomarker study for kidney injury induced by immune complex

作　　者：林志[1,2] 张颖丽[2] 吕建军[2] 屈哲[2] 张頔[2] 杨艳伟[2] 李珊珊[2] 李波[2] 孙建宁[1] 

机构地区：[1]北京中医药大学,北京100029 [2]中国药品生物制品检定所

出　　处：《毒理学杂志》2012年第3期165-169,共5页Journal of Toxicology

摘　　要：目的通过构建免疫复合物肾小球肾炎动物模型,探讨C3aR和STAT3因子作为免疫复合物肾脏损伤的生物标记物辅助诊断的意义。方法 BALB/c小鼠腹腔注射HSA和生理盐水,采用组织病理学、电子显微镜以及免疫组织化学等方法进行研究。结果 HSA组动物表现出免疫复合物肾小球肾炎的变化,并且C3aR、STAT3和p-STAT3因子的表达明显高于阴性对照组。结论 C3aR、STAT3和p-STAT因子可能参与了免疫复合物性肾小球肾炎的发生,有望成为免疫复合物肾脏损伤辅助诊断的生物标记物。Objective To investigate the mechanism of immune complex glomerulonephritis and discuss the significance of C3aR,STAT3 and p-STAT3 as biomarkers for immune complex glomerulonephritis.Methods HSA（Horse spleen apoferritin） and sodium chloride were intraperitoneally（ip） administrated to BALB/c mice.Pathology examination,electron microscope examination,and immunohistochemistry of C3aR,STAT3 and p-STAT3 were used for pathology evaluation of kidney injury.Results Many changes of immune complex glomerulonephritis were found in HSA group animals.Compared with the control group,the expressions of C3aR,STAT3 and p-STAT3 were increased in kidney of HSA group animals by immunohistochemistry examination.Conclusion C3aR,STAT3 and p-STAT3 should be involved in immune complex glomerulonephritis induced by HAS,and they may become the important biomarkers for the diagnosis of immune complex glomerulonephritis.

关 键 词：免疫复合物 肾小球肾炎 生物标记物 

分 类 号：R991[医药卫生—毒理学]