EGFR抑制剂AG1478对胶质瘤C6细胞增殖及凋亡的影响  

Effect of EGFR inhibitor AG1478 on the proliferation and apoptosis of glioma C6 cell line

作　　者：李军[1] 杨梅[2] 李伟[3] 徐宏昌[4] 

机构地区：[1]河北大学医学实验中心,河北保定071000 [2]河北大学医学培训中心 [3]河北大学教务处 [4]河北大学图书馆

出　　处：《山东医药》2012年第26期14-16,共3页Shandong Medical Journal

基　　金：河北省中医药管理局科研计划项目(2009150)

摘　　要：目的观察表皮生长因子受体(EGFR)抑制剂AG1478对胶质瘤C6细胞增殖和凋亡的影响。方法 C6细胞中分别加入5、10、25、50μmol/L的AG1478,作用48 h。用MTT法测算C6细胞生长抑制率,倒置显微镜观察细胞形态学的改变,流式细胞术测算细胞凋亡率。结果 AG1487能够抑制C6细胞的增殖,其抑制作用呈剂量—效应依赖关系。在50μmol/L的AG1478作用下,C6细胞变圆、细胞突起减少,贴壁瘤细胞数量明显减少,排列稀疏。25μmol/L的AG1478作用C6细胞48 h,细胞凋亡率显著增加。结论 EGFR抑制剂AG1478可抑制胶质瘤C6细胞的增殖,促进细胞凋亡。objective To investigate the effect of epidermal growth factor receptor （EGFR） tyrosine kinase inhibitors （AG1478） on cell proliferation and apoptosis of glioma cell C6 in vitro. Methods Cell proliferation was detected by methyl thiazolyl tetrazolium（MTr）. The morphologic changes of C6 cell were observed with inverted microscope. The changs of tumor cell apoptosis were detected by flow cytometry. Results Compared with the control group, different concentra- tions of AG1478 inhibited the proliferation of glioma cells significantly. The growth of the C6 cell lines was significantly suppressed in a dose-dependent manner. Under inverted microscope, some shrinkages of cytoplasm of C6 cell and exfoliated cells as well as the ammounts of glioma cells were found after treatment with 50 μmol/L concentration of AG1478. After treatment with 25 μmol/L concentration of AG1478 for 48 hours, the apoptosis ratio was increased compared with control group. Conclusions EGFR inhibitor AG1478 has effective inhibiting function in the proferation of glima C6 cell strain in vitro, the mechanism may be connected with its process of cell apoptosis.

关 键 词：表皮生长因子受体 酪氨酸激酶抑制剂 细胞凋亡 细胞增殖 脑肿瘤 胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]