转染pIRESneo-EGFP-BDNF的骨髓间充质干细胞侧脑室注射对帕金森大鼠行为学的影响  

Influence on behavior of rats with Parkinson′s disease treated by bone mesenchymal stem cell modified by plasmid pIRESneo-EGFP-BDNF

作　　者：张平[1] 赵钢勇[2] 宋月平[1] 苏立凯[1] 

机构地区：[1]河北大学附属医院,河北保定071000 [2]河北大学生物工程技术研究中心

出　　处：《山东医药》2012年第26期23-26,共4页Shandong Medical Journal

基　　金：河北省科学技术研究与发展计划项目(08206120D)

摘　　要：目的观察转染pIRESneo-EGFP-BDNF的骨髓间充质干细胞(MSCs)侧脑室注射对帕金森病(PD)大鼠行为学的影响。方法将pEGFP(N1)-BDNF与pIRESneo进行双酶切后再连接,构建高拷贝质粒pIRESneo-EGFP-BDNF,采用电穿孔法将其转染MSCs。采用6-羟多巴(6-OHDA)制备PD大鼠模型(36只),造模成功后将其随机分为3组,每组12只。B组为模型组:侧脑室注射生理盐水;C组:侧脑室注射MSCs;D组:侧脑室注射转染pIRESneo-EGFP-BDNF的MSCs;A组(12只大鼠,为假手术组):以生理盐水代替6-OHDA后,侧脑室注射生理盐水。分别于侧脑室注射后2、48、周腹腔注射阿扑吗啡(APO)诱导大鼠旋转,观察各组大鼠行为学变化。结果经双酶切鉴定,pIRESneo-EGFP-BDNF构建成功。侧脑室注射细胞干预PD大鼠模型后2、4、8周,大鼠旋转次数D组<C组<B组(P均<0.05);D组大鼠旋转次数明显减少,但仍较A组多。结论侧脑室注射转染pIRESneo-EGFP-BDNF的MSCs能明显改善PD大鼠的行为能力。Objective To observe influence on behavior of Parkinson＇s disease （PD） model treated by bone mesenchymal stem cells （MSCs） modified by plasmid pIRESneo-EGFP-BDNF. Methods pEGFP-BDNF and pIRESneo were double enzymed and then were reconstructed into pIRESneo-EGFP-BDNF which was transfected to MSCs with electroproa- tion. 36 rat models of PD were set up by 6-OHDA and divided into three groups randomly; group B（ group model） ： intracerebroventricular injection of saline ; group C ： intraeerebroventricular injection of bone MSCs ; group D ： intracerebroventricular injection of bone MSCs modefied by pIRESneo-EGFP-BDNF. Group A （12 rats, sham opreation groups ） ： saline instead of 6-OHDA, than intracerebroventricular injection of saline. The rotating behavior of rat models induced by Apomorphine intraperitoneally which transplanting bone MSCs or MSCs modified by plasmid pIRESneo-EGFP-BDNF through cerebral lateral ventricle after 2, 4 and 8 weeks. Results Plasmids pIRESneo-EGFP-BDNF were constructed successfully, which was identified by double digestion. The rotation numbers （r/min） of D group 〈 C group 〈 B group in the 2th, 4th and 8th week after transplanting were significant decrease compared with PD group （ all P 〈 0.05）. Those of D group were exespecially significant decreased, but were increased compared with A group. Conclusion The behavior of rat with PD was improved significantly while transplanting bone MSCs modified by BDNF through cerebral lateral ventricle.

关 键 词：帕金森病 骨髓间充质干细胞 脑源性神经生长因子 质粒 侧脑室注射 大鼠 

分 类 号：R741[医药卫生—神经病学与精神病学]