亚硫酸钠预处理提高稻草酶水解糖化效率的研究  

Effects of Sodium Sulfite Pretreatment on Improving Saccharification Efficiency of Rice Straw

作　　者：曹杰[1] 张帅[1] 杨林峰[1] 王旺霞[1] 吴文娟[1] 金永灿[1] 

机构地区：[1]南京林业大学江苏省制浆造纸科学与技术重点实验室,江苏南京210037

出　　处：《纤维素科学与技术》2012年第2期1-10,共10页Journal of Cellulose Science and Technology

基　　金：引进国际先进林业科学技术项目(2012-4-18);国家自然科学基金资助项目(31070512);江苏省高校自然科学研究重大项目(10KJA22020)

摘　　要：研究了亚硫酸钠预处理对稻草化学组分变化及酶水解性能的影响。结果表明,提高温度或增加Na2SO3用量可以脱除更多的木质素和半纤维素,酶水解效率也相应提高,但木质素脱除率达到50%以后,继续增强预处理条件,对酶水解糖得率无显著的促进作用。相比而言,加大Na2SO3用量更有利于使木质素溶出,提高温度更有利于使高聚糖溶出,加大Na2SO3用量比提高温度对酶水解效率的提高影响更显著。通过实验得到亚硫酸钠预处理稻草的最优条件,在温度为140℃,Na2SO3用量为16%,纤维素酶用量为20 FPU/g(对纤维素)时,总糖转化率达到最大,为74.9%,此时的总糖得率为43.5%。The effects of sodium sulfite （Na2SO3） pretreatment on chemical composision and enzymatic hydrolysis of rice straw were investigated. Raising the temperature or increasing the charge of sodium sulfite can improve the dissolution of lignin and saccharification efficiency, while most carbohydrate is retained. However, when the dissolution of lignin attained 40%, the improvement of saccharification efficiency was nonsignificant. After enzymatic hydrolysis, total sugar conversion rate of 74.9% and total sugar yield rate of 43.5% can be achieved by using the substrate pretreated under the condition of temperature 140~C, sodium sulfite charge 16% and cellulase amount 20 FPU/g （cellulose）.

关 键 词：稻草 Na2SO3预处理 化学成分 酶水解 糖转化率 

分 类 号：TQ352.62[化学工程]