急性髓细胞白血病中胰岛素样生长因子2基因印迹的研究  

Loss of imprinting of insulin-like growth factor Ⅱ gene in acute myeloid leukemia

作　　者：刘永忠[1] 杨本海[1] 祝怀平[2] 汪健[2] 

机构地区：[1]安徽省皖南医学院附属医院临床医学研究所,芜湖241001 [2]安徽省立医院

出　　处：《中华医学遗传学杂志》2000年第1期39-41,共3页Chinese Journal of Medical Genetics

摘　　要：目的　了解急性髓细胞白血病(acutemyeloidleukemia,AML)中胰岛素样生长因子2(insulinlikegrowthfactor2,IGF2)基因的印迹状态。方法　根据IGF2基因第9外显子具有Apa位点多态性,利用聚合酶链反应(PCR)结合限制性长度多态性技术,对26例AML患者骨髓细胞IGF2基因进行杂合子筛选,并进一步检测杂合子个体该基因的印迹丢失(lossofimprinting,LOI)。结果　11例AML患者为杂合子信息个体(1126),其中8例为双等位基因表达,即发生了LOI(811)。结论　IGF2基因的印迹丢失可能参与急性髓细胞白血病的发生和发展。Objective To investigate the status of insulin like growth factor Ⅱ(IGF 2) gene imprinting in acute myeloid leukemia(AML).Methods Selection of heterozygous cases for Apa Ⅰpolymorphism within exon 9 of IGF 2 gene and further analysis of loss of imprinting(LOI) were done in 26 AML patients by polymerase chain reaction(PCR),reverse transcription PCR(RT PCR) and restriction fragment length polymorphism(RFLP) technique.Results Eleven cases of AML patients were identified as heterozygous for IGF 2(11/26);8 heterozygous patients showed biallelic expression of IGF 2,indicating loss of IGF 2 imprinting.Conclusion The frequent LOI of IGF 2 may play a role in the development of acute myeloid leukemia.

关 键 词：印迹丢失 白血病 AML IGF-2 

分 类 号：R733.71[医药卫生—肿瘤]