Comparison of Lipopolysaccharide and Protein Immunogens from Pathogenic Yersinia enterocolitica Bio-serotype 1B/O:8 and 2/O:9 using SDS-PAGE  

作　　者：GU Wen Peng WANG Xin QIU Hai Yan LUO Xia XIAO Yu Chun TANG Liu Ying KAN Biao XU Jian Guo JING Huai Qi 

机构地区：[1]National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,State Key Laboratory Jbr Infectious Disease Prevention and Control Beijing 102206,China [2]National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China

出　　处：《Biomedical and Environmental Sciences》2012年第3期282-290,共9页生物医学与环境科学（英文版）

基　　金：supported by National Natural Science Foundation of China (General Project,No. 30970094);National Sci-Tech Key Project (2009ZX10004-201,2009ZX10004-203)

摘　　要：Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitico. Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide （LPS）, Yersinio outer membrane proteins （Yops）, membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitico bio-serotype 2/0：9, isolated from China, and highly pathogenic bio-serotype 1B/O：8, isolated from Japan. Results These two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains. Conclusion The major antigens of the two strains e and membrane proteins, as shown by comparing preparations. citing the host immune protein samples with response were the LPS reference and purifiedObjective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitico. Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide （LPS）, Yersinio outer membrane proteins （Yops）, membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitico bio-serotype 2/0：9, isolated from China, and highly pathogenic bio-serotype 1B/O：8, isolated from Japan. Results These two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains. Conclusion The major antigens of the two strains e and membrane proteins, as shown by comparing preparations. citing the host immune protein samples with response were the LPS reference and purified

关 键 词：Immunogens LIPOPOLYSACCHARIDES Outer membrane proteins Whole-cell proteins Pathogenic Yersinia enterocolitica 

分 类 号：S512.1[农业科学—作物学] Q939.3[生物学—微生物学]