Dimethoate Induced Oxidative Damage and Histopathological Changes in lung of Adult rats:Modulatory Effects of Selenium and/or Vitamin E  

作　　者：Ibtissem Ben AMARA Nejla SOUDANI Afef TROUDI Ahmed HAKIM Khaled Mounir ZEGHAL Tahia BOUDAWARA Najiba ZEGHAL 

机构地区：[1]Animal Physiology Laboratory,Faculty of Science,BP1171,3000 Sfax,University of Sfax,Tunisia [2]Laboratory of Pharmacology,Faculty of Medicine,3029 Sfax,University of Sfax Tunisi [3]Anatomopathology Laboratory,CHU Habib Bourguiba 3029 Sfax,University of Sfax,Tunisia

出　　处：《Biomedical and Environmental Sciences》2012年第3期340-351,共12页生物医学与环境科学（英文版）

摘　　要：Objective To determine the efficiency of selenium and/or vitamin E to alleviate lung oxidative damage induced by dimethoate, an organophosphorus compound. Methods Adult Wistar rats were exposed during 30 days either to dimethoate （0.2 g/L of drinking water）, dimethoate＋selenium （0.5 mg/kg of diet）, dimethoate＋vitamin E （100 mg/kg of diet）, or dimethoate＋selenium＋vitamin E. Results Exposure to dimethoate caused oxidative stress in lung evidenced by an increase of malondialdehyde, protein carbonyl groups and advanced oxidation protein products. An increase in glutathione peroxidase, superoxide dismutase, catalase and a decrease in acetylcholinesterase and butyrylcholinesterase activities, glutathione, non-protein thiols and vitamins C levels were observed. Histopathological changes in lung tissue were noted as emphysema, hemorrhages and hemosiderin deposits. Co-administration of selenium or vitamin E to the diet of dimethoate treated rats ameliorated the biochemical parameters as well as histological impairments. The joint effect of these elements was more powerful in antagonizing dimethoate-induced lung oxidative damage. Conclusion We concluded that selenium and vitamin E ameliorated the toxic effects of this pesticide in lung tissue suggesting their role as potential antioxidants.Objective To determine the efficiency of selenium and/or vitamin E to alleviate lung oxidative damage induced by dimethoate, an organophosphorus compound. Methods Adult Wistar rats were exposed during 30 days either to dimethoate （0.2 g/L of drinking water）, dimethoate＋selenium （0.5 mg/kg of diet）, dimethoate＋vitamin E （100 mg/kg of diet）, or dimethoate＋selenium＋vitamin E. Results Exposure to dimethoate caused oxidative stress in lung evidenced by an increase of malondialdehyde, protein carbonyl groups and advanced oxidation protein products. An increase in glutathione peroxidase, superoxide dismutase, catalase and a decrease in acetylcholinesterase and butyrylcholinesterase activities, glutathione, non-protein thiols and vitamins C levels were observed. Histopathological changes in lung tissue were noted as emphysema, hemorrhages and hemosiderin deposits. Co-administration of selenium or vitamin E to the diet of dimethoate treated rats ameliorated the biochemical parameters as well as histological impairments. The joint effect of these elements was more powerful in antagonizing dimethoate-induced lung oxidative damage. Conclusion We concluded that selenium and vitamin E ameliorated the toxic effects of this pesticide in lung tissue suggesting their role as potential antioxidants.

关 键 词：DIMETHOATE SELENIUM Vitamin E RATS LUNG Antioxidant status Histopathological studies 

分 类 号：R151[医药卫生—营养与食品卫生学]