Comparative Analyses of H3K4 and H3K27 Trimethylations Between the Mouse Cerebrum and Testis  

作　　者：Peng Cui Wanfei Liu Yuhui Zhao Qiang Lin Daoyong Zhang Feng Ding Chengqi Xin Zhang Zhang Shuhui Song Fanglin Sun Jun Yu Songnian Hu 

机构地区：[1]CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing 100029,China [2]Institute of Epigenetics and Cancer Research,School of Medicine,Tsinghua University,Beijing 100080,China [3]Computational Bioscience Research Center,4700 King Abdullah University of Science and Technology,Thuwa123955-6900,Kingdom of Saudi Arabia [4]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Genomics, Proteomics & Bioinformatics》2012年第2期82-93,共12页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by Grants from Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-EW-R-01-04);National Science and Technology Key Project (2008ZX1004-013);863 Program(2009AA01A130);Special Foundation Work Program(2009FY120100);National Key Technology R&D Program (2008BA164B02);973 Program (2011CB944100,2011CB965300 and 2007CB948101) from the Ministry of Science and Technology of the People’s Republic of China

摘　　要：The global features of H3K4 and H3K27 trimethylations （H3K4me3 and H3K27me3） have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genorne-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChlP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLID technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. How- ever, the bivalent domains tend towards a ＂winner-takes-all＂ approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a ＂winner-takes-all＂ principle to regulate gene expression.The global features of H3K4 and H3K27 trimethylations （H3K4me3 and H3K27me3） have been well studied in recent years, but most of these studies were performed in mammalian cell lines. In this work, we generated the genorne-wide maps of H3K4me3 and H3K27me3 of mouse cerebrum and testis using ChlP-seq and their high-coverage transcriptomes using ribominus RNA-seq with SOLID technology. We examined the global patterns of H3K4me3 and H3K27me3 in both tissues and found that modifications are closely-associated with tissue-specific expression, function and development. Moreover, we revealed that H3K4me3 and H3K27me3 rarely occur in silent genes, which contradicts the findings in previous studies. Finally, we observed that bivalent domains, with both H3K4me3 and H3K27me3, existed ubiquitously in both tissues and demonstrated an invariable preference for the regulation of developmentally-related genes. How- ever, the bivalent domains tend towards a ＂winner-takes-all＂ approach to regulate the expression of associated genes. We also verified the above results in mouse ES cells. As expected, the results in ES cells are consistent with those in cerebrum and testis. In conclusion, we present two very important findings. One is that H3K4me3 and H3K27me3 rarely occur in silent genes. The other is that bivalent domains may adopt a ＂winner-takes-all＂ principle to regulate gene expression.

关 键 词：H3K4me3 H3K27me3 MOUSE 

分 类 号：Q492.4[生物学—生理学] Q983.5