HB柚有性后代的杂种鉴定和遗传多样性分析  

Identification and genetic diversity analysis of hybrid progenies from HB pummelo

作　　者：杨海健[1] 伊华林[1] 

机构地区：[1]华中农业大学园艺林学学院,武汉430070

出　　处：《华中农业大学学报》2012年第5期574-577,共4页Journal of Huazhong Agricultural University

基　　金：国家现代柑橘产业技术体系专项

摘　　要：以HB柚为母本,与江西无核椪柑杂交,获得380株杂交后代。筛选出5对SSR引物对其中的90株后代进行检测,杂种率为97.8%;杂种后代中出现了部分条带缺失和新条带。采用UPGMA聚类分析法分析后代群体和亲本的遗传关系,相似系数为0.720时,可将杂种后代分为4类,而与父母本聚在一起的杂种植株占少数。Five pairs SSR primers was selected to identify 90 offsprings obtained from crossing HB pomelo（Citrus grandis Osbeck） with Jiangxi seedless Ponkan（C. reticulate Blanco）. The rate of true hy- brid was 97. 8%. There were lost and novel bands in some of the hybrids. The genetic relationship be- tween parents and offsprings was analyzed with UPGMA. The hybrids could be divided into four groups with similarity coefficient of 0. 720 and only few progenies were clustered with parents. These created germplasms provided new materials for the citrus cultivar improvement.

关 键 词：HB柚 杂交育种 杂种鉴定 SSR 遗传多样性 UPGMA聚类分析 

分 类 号：S666.3[农业科学—果树学]