胃癌病人转化生长因子β1及其受体检测的意义  

Clinical significance of the measurement of transforming growth factor levels in patients with gastric carcinoma

作　　者：许洪卫[1] 王元和[1] 潭龙益[2] 

机构地区：[1]中国人民解放军第二军医大学长征医院普通外科,上海200003 [2]中国人民解放军第二军医大学长征医院检验科,上海200003

出　　处：《中华普通外科杂志》2000年第2期72-74,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金!(基金号 395 70 786)

摘　　要：目的　通过检测胃癌病人血清转化生长因子β1浓度和组织转化生长因子βⅡ型受体的mRNA水平 ,探讨它们与胃癌浸润和转移的关联性。方法　应用酶联免疫吸附法测定 2 9例胃癌病人和 3 4例对照者的血清转化生长因子 β1浓度 ;应用点杂交法和计算机灰度扫描分析组织的mRNA灰度均值。结果　胃癌组的血清转化生长因子 β1浓度显著高于对照组 (P <0 0 1) ,分化较差组、浸润组及伴有淋巴结转移组均分别明显高于分化较好组、膨胀组及无淋巴结转移组 (P <0 0 1～ 0 0 5 ) ;各组的转化生长因子 βⅡ型受体mRNA水平变化却与转化生长因子 β1的相反 ,二者具有明显的负相关性(r=- 0 74 2 ,P<0 0 5 )。结论　血清转化生长因子 β1和组织转化生长因子 βⅡ型受体可能在胃癌的浸润和转移过程中发挥某种不同的作用。Objectives In this study, serum transforming growth factor β1(TGF β1) concentration and tissue transforming growth factor β type II receptor (TGF β RⅡ) mRNA level in 29 cases of gastric carcinoma were measured to detect their relationship with the aggressiveness and metastasis of the malignancy. Methods Fasting concentration of serum TGF β1 was determined by ELISA,and expression of TGF β1 mRNA in the tissue was analyzed by dot blot and computer scanning. Results Serum TGF β1 concentration was much higher ( P <0 01) in gastric cancer patients than that of healthy controls, and it also was significantly higher ( P <0 05) in patients with poor differentiation, invasive type or metastatic lymphy nodes,than those with well differentiation, the expansive growth or without metastatic lymphy nodes, respectively.Whereas changes of tissue TGF β RⅡ mRNA among all groups were in a reverse relationship to those of serum TGF β1 concentration. Moreover, gray value of TGF β RⅡ mRNA was negatively correlated with those of TGF β1 (r=-0 742, P <0 05). Conclusion Serum TGF β1 and tissue TGF β RⅡ may play a contrary role on the invasive and metastatic progression of gastric carcinomas.

关 键 词：胃肿瘤 转化生长因子 肿瘤转移 TGFΒ1 受体 

分 类 号：R735.2[医药卫生—肿瘤]