胶质瘤bcl-2基因表达水平与其细胞增殖和凋亡关系的研究  

Relationship of bcl-2 gene expression with cell proliferation and apoptosis in human gliomas

作　　者：于士柱[1] 浦佩玉[1] 江德华[1] 安同岭[1] 管欣琴[1] 杨露春[1] 

机构地区：[1]天津医科大学总医院,天津市神经病学研究所300052

出　　处：《中华病理学杂志》2000年第1期12-15,共4页Chinese Journal of Pathology

基　　金：天津市教委重点学科科研基金资助项目

摘　　要：目的　探讨胶质瘤细胞bcl 2基因表达水平与肿瘤恶性程度、细胞增殖活性及凋亡程度的关系。方法　以 6 9例不同级别的人胶质瘤组织为研究对象 ,用原位杂交及免疫组化染色ABC法分别检测bcl 2mRNA、bcl 2蛋白和增殖细胞核抗原 (细胞增殖活性标记物 )的表达 ,并用 3′末端标记法做原位细胞凋亡检测。结果　 6 4例 (92 .8% )表达bcl 2mRNA ,6 0例 (87.0 % )表达bcl 2蛋白 ,两者的表达水平呈正相关 (rs=0 .999,P <0 .0 1) ,并均显示Ⅰ～Ⅱ级组低于Ⅲ级组 ,Ⅳ级组最高 (P <0 .0 2～ 0 .0 0 1)。随肿瘤细胞bcl 2蛋白表达水平升高其增殖活性相应增加 ,凋亡相应减少 ,bcl 2蛋白 +++组与 ++组间 (P <0 .0 5 )及前两组分别与 -组和 +组间 (P <0 .0 1)这两种指标的差异均有显著性。结论　胶质瘤细胞bcl 2基因高表达可抑制其凋亡 ,可能由此引起的细胞凋亡减少与其他基因异常所致的细胞过度增殖共同导致细胞无限蓄积 ,并在胶质瘤发生。Objective To investigate the relationship of bcl 2 gene expression level in human gliomas with the malignant degree, cell proliferative activity and apoptosis of the tumors. Methods The expression of bcl 2 mRNA, bcl 2 protein and proliferating cell antigen, and the apoptosis in sixty nine human glioma specimens with different malignant grades were studied using in situ hybridization, in situ cell death detection (TUNEL method) and immunohistochemistry. Results Of the 69 gliomas, 64 (92.8%) and 60 (87.0%) expressed bcl 2 mRNA and bcl 2 protein, respectively. The expression levels of bcl 2 mRNA and bcl 2 protein were correlated positively with each other ( r s =0.999, P <0.01). The expression levels of bcl 2 mRNA and bcl 2 protein were both higher in WHO grade IV gliomas than in grade III gliomas, and the expression was lowest in grade Ⅰ Ⅱ gliomas ( P <0.02～0.001). With the increase in the expression of bcl 2 protein, the cell proliferating activity increased and apoptosis decreased in the tumor cells. There was significant difference of cell proliferation and apoptosis between +++ group and ++ group of bcl 2 protein expression ( P <0.05) as well as between both the former groups, and the negative and + group ( P <0.01) respectively. Conclusions Over expression of bcl 2 gene inhibits apoptosis of glioma cells, and the inhibitory intensity increases with the ascending of bcl 2 gene expression level in glioma cells. Both the decrease in apoptosis caused by bcl 2 gene over expression and the excessive cell proliferation promoted by other gene abnormalities may result in unlimited cell accumulation, which may play an important role in the development and malignant progression of gliomas.

关 键 词：神经胶质瘤 BCL-2基因 细胞增殖 细胞凋亡 

分 类 号：R730.2[医药卫生—肿瘤]