线粒体DNA片段诱导小鼠胚胎成纤维细胞恶性转化  

Malignant transformation of mouse embryonic fibroblast induced by mitochondrial DNA fragments

作　　者：胡义德[1] 钱桂生[1] 毛宝龄[1] 肖桃元[2] 李懿[2] 曹世龙[3] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,重庆400037 [2]第三军医大学新桥医院,重庆400037 [3]上海医科大学

出　　处：《中华病理学杂志》2000年第1期39-42,共4页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目!(39500173)

摘　　要：目的　探讨线粒体DNA(mtDNA)片段的恶性转化效应和转化机制。方法　采用基因转入技术 ,观察经mtDNA片段转染后小鼠胚胎成纤维细胞 (NIH3T3)在裸小鼠体内的成瘤能力 ,并对形成的肿瘤进行病理观察 ;同时应用荧光原位杂交 (FISH)方法 ,观察mtDNA片段在NIH3T3细胞核内的整合情况。结果　转染后 1周 ,18%～ 2 0 %的NIH3T3细胞核中发现有目的基因探针的阳性杂交信号 ;转染后 2周的培养细胞在裸小鼠皮下形成肿瘤的能力为 10 0 % (8 8) ;且形成肿瘤的病理表现与纤维肉瘤的特征相一致。Objective To investigate the malignant transforming effect and mechanism of mitochondrial DNA (mtDNA) fragments. Methods Tumorigenicity of mtDNA transformed mouse embryonic fibroblast (NIH3T3) in nude mice was studied using transgenic techniques. Transformed tumors were detected by pathological examination and hybridization signals of mtDNA probe were analyzed by fluorescence in situ hybridization (FISH) techniques. Results Hybridization signals were observed on the nuclei of 18%～20% NIH 3T3 cells 1 week after mtDNA fragments transforming. Tumor from mtDNA transformed NIH 3T3 cells was developed in all 8 nude mice (8/8) respectively 2 weeks after the transformation. The pathological characteristics of the tumors developed were similar to that of fibrosarcoma. Conclusions Auto integration of mtDNA fragments into nuclear genome is a new factor involved in carcinogenesis.

关 键 词：DNA 线粒体 成纤维细胞 小鼠 癌变 

分 类 号：R73-3[医药卫生—肿瘤]