金黄地鼠实验性肺气肿肺泡Ⅱ型上皮表面活性物质蛋白A和B的变化  

Changes of surfactant A and B in alveolar type Ⅱ cells in hamster with elastase induced emphysema

作　　者：韩晓男[1] 阮英茆[1] 张旭晨[1] 王红月[1] 褚燕 

机构地区：[1]中国医学科学院中国协和医科大学阜外心血管病医院病理研究室,北京100037

出　　处：《中华病理学杂志》2000年第1期43-45,共3页Chinese Journal of Pathology

基　　金："九五"国家科技攻关基金资助项目!(969060216)

摘　　要：目的　观察金黄地鼠实验性肺气肿肺泡Ⅱ型上皮表面活性物质蛋白 (SP)A和B的变化。方法　金黄地鼠气管注入弹性蛋白酶后 ,分别于注入弹性蛋白酶的 30、6 0和 90d处死 ,肺组织经光镜检查和图像分析后 ,再行SP A和SP B免疫组化染色和形态定量 ;同时进行透射电镜观察。结果　注入弹性蛋白酶后第 30、6 0和 90d肺平均内衬间隔明显增大 (P <0 .0 1) ;免疫组化显示注入弹性蛋白酶后 30d肺泡Ⅱ型上皮细胞SP A和SP B阳性率分别由 (48.0± 3.0 ) %降至 (9.5± 4.0 ) %、(2 8.0±4.0 ) %降至 (13.3± 4.1) % ,P <0 .0 1,但随时间延至 90d而有所恢复 ;并且与肺气肿程度呈明显负相关。电镜观察发现 ,注入弹性蛋白酶后 ,Ⅱ型上皮细胞内板层小体数减少。结论　实验性金黄地鼠肺气肿肺泡Ⅱ型上皮细胞内SP A和SP B明显减少可能是肺气肿发展的重要环节之一。Objective To observe the changes of surfactant A and B (SP A and SP B) in alveolar type II cells of lungs in hamsters with elastase induced emphysema. Methods After intratracheal instilling of elastase, hamsters were killed at the 30th, 60th and 90th day of the experiment respectively. Slides of lung tissue were examined under light microscope and measured by calculating the mean linear intercepts. Then the slides were stained under immunohistochemistry procedures and measured by using a morphometric analysis system. Meanwhile, slides were also checked by electron microscopy. Results In comparison with the normal group, MLI of the elastase group was significantly increased ( P <0.01). Immunohistochemistry showed that the percentage of both SP A and SP B positive cells was significantly decreased 30 days after the intratracheal instilling of elastase (from 48.0%±3.0% to 9.5%±4.0% and from 28.0%±4.0% to 13.3%±4.1%, respectively; P <0.01), however, a time-dependent recovery 90 days after instilling was seen. Electron microscopy showed a significantly decrease of the number of lamellae. Conclusion The decrease of SP A and SP B in alveolar type II cells seems to play an important role in the development of emphysema.

关 键 词：肺气肿 上皮细胞 肺表面活性剂 肺泡 SP 

分 类 号：R363[医药卫生—病理学]