木聚糖酶产生菌的筛选与酶学生化特性  

Isolation of xylanase-producing microbes and analysis of its biochemical properties

作　　者：李淑丽[1] 王娇[1] 尹清强[1] 常娟[1] 左瑞雨[1] 王平 王潇 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]河南普爱饲料股份有限公司,周口466000

出　　处：《生物加工过程》2012年第4期54-58,共5页Chinese Journal of Bioprocess Engineering

基　　金：河南省科技成果转化项目(082201180001)

摘　　要：从自然界中筛选出可分泌高产木聚糖酶的黑曲霉,并对其产酶条件和酶学特性进行优化和分析。结果表明:该菌株培养48 h后,所产的木聚糖酶比酶活最高,达到1 645.66 U/g;通过培养基的优化,其比酶活达到了2 698.28 U/g。最优培养基组成(质量分数):麸皮75.0%、玉米12.5%、豆粕12.5%。该酶的最适温度为37℃,最适pH为4.5。The microbes with high levels of xylanase production were selected.The results showed that Aspergillus niger,xylanase was isolated with the high activity of 1 645.66 U/g at 48 h cultivatiion（P〈0.05）.Xylanase activity reached 2 698.28 U/g after medium selection（P〈0.05）.The optimal medium composition（in mass）was wheat bran 75.0%,corn 12.5%,and soybean meal 12.5%.The optimal temperature and pH were 37 ℃ and 4.5,respectively（P〈0.05）.

关 键 词：黑曲霉 培养基 PH 温度 木聚糖酶 

分 类 号：Q556[生物学—生物化学]