马桑内酯致痫大鼠大脑皮层、海马NMDA受体亚单位1(NMDAR1)mRNA表达的变化  

The alterations of NMDA receptor subunit 1 mRNA in rat cerebral cortex and hippocampus of seizure rats induced by coriaria lactone

作　　者：王群[1] 阮旭中[2] 史庭慧[2] 张苏明[2] 

机构地区：[1]北京天坛医院神经内科 [2]同济医科大学同济医院神经内科,湖北武汉430030

出　　处：《中风与神经疾病杂志》2000年第1期17-19,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金重点项目资助课题!(39330210)

摘　　要：目的　探讨癫痫发病的分子机制。方法　采用原位杂交技术 ,研究了马桑内酯致痫大鼠大脑皮层、海马 N-甲基 - D-天门冬氨酸受体亚单位 1(NMDAR1) m RNA表达的变化。结果　马桑内酶致痫大鼠顶叶大脑皮层及海马齿状回 NMDAR1m RNA水平显著高于生理盐水对照组 (P<0 .0 1,P<0 .0 5 )。结论　马桑内酯上调脑组织内NMDAR1m RNA水平 。Objective In order to clarify further molecular pathological mechanisms of epilepsy induced by coriaria lactone(CL). Methods We investigated the alterations of N methyl D aspartate receptor 1 (NMDAR1) mRNA in rat cerebral cortex and hippocampus by using digoxigenin labled cDNA probe,nonradioactive in situ hybridization technique. Results It is found that NMDAR1 mRNA level in parietal cortex(PC) and dentate gyrus(DG) of the hipocampus in seizure rats induced by CL was higher than those of NS control group (P<0.01,P<0.05). Conclusion We concluded that CL upregulated NMDAR1 subunit mRNA level and be contributed to the proconvulsant and increased seizure susceptibility molecular mechnisms of CL.

关 键 词：马桑内酯 癫痫 mRNA NMDAR1 大鼠 

分 类 号：R742.102[医药卫生—神经病学与精神病学]