四氢小檗碱对β-AP诱导神经元凋亡的影响  

Effects or Tetrohydroberberine on Neuron Apoptosis Induced by β-amyloid Peptides

作　　者：许长庆[1] 吴琪[1] 钱采韵[1] 

机构地区：[1]中山医科大学附属第一医院神经科,510080

出　　处：《中国临床神经科学》2000年第1期47-49,共3页Chinese Journal of Clinical Neurosciences

摘　　要：目的：阐明β－ＡＰ诱导神经元凋亡的机制及四氢小檗碱的保护作用。方法：１．形态学观察β－ＡＰ２５－３５诱导离体海马培养神经元凋亡；２．玻片法培养海马神经元，分６组观察四氢小檗碱的保护作用；３．用ＭＴＴ法检测细胞存活情况；４．用Ｆｕｒａ－２技术测定细胞内游离Ｃａ２＋含量。结果：四氢小檗碱组的海马神经元凋亡数量均显著降低（Ｐ＜０．０５），及明显降低β－ＡＰ可诱导细胞内Ｃａ２＋增高，并呈剂量依赖性。结论：四氢小檗碱对神经元凋亡有保护作用，可能与降低细胞内Ｃａ２＋含量有关。Aim: To elucidate the mechanism of neuron apoptosis induced by β-AP and the protective effects of tetrohy- droberberine. Methods: 1. Apoptosis of free hippocampal cultured neurons induced by β-AP25-35 was observed morphologi- cally. 2. Hippocampal neurons were cultured by glass method and divided into six groups to observe the protective effects of tetrohydroberberine. 3. The conditions of cell survival were determined by MTT method. 4. Concentrations of free Ca2+ in cells were assayed by Fura-2 technology. Results: Apoptosis numbers of hippocampal neurons in tetrohydrobergerine groups decreased significantly (P＜0. 05) and the increase of Ca2+ concentration in β-AP inducible cells reduced significantly and dose-dependently. Conclusion: Tetrohydroberberine protects neurons from apoptosis induced by β-AP, which may be associat- ed with the decrease of Ca2+ concentration in cells.

关 键 词：四氢小檗碱 神经元凋亡 Β-AP 早老性痴呆 

分 类 号：R749.16[医药卫生—神经病学与精神病学]