遗传性非息肉病性大肠癌FasL蛋白表达与肿瘤浸润淋巴细胞数量及分布的关系  

Expression of FasL Protein in Hereditary Nonpolyposis Colorectal Cancer and Its Relationship with Tumor-infiltrating Lymphocyte Number and Distribution

作　　者：李洁明[1] 郑林辉[2] 李太原[2] 

机构地区：[1]江西省人民医院普外科 [2]南昌大学第一附属医院普外科,南昌330006

出　　处：《南昌大学学报（医学版）》2012年第5期24-27,F0003,共5页Journal of Nanchang University：Medical Sciences

摘　　要：目的探讨遗传性非息肉病性大肠癌(hereditary nonpolyposis colorectal cancer,HNPCC)肿瘤浸润淋巴细胞(Tumor infiltrating lymphocyte,TIL)计数高于散发性大肠癌(sporadic colorectal carcinoma,SCRC)可能的原因。方法应用免疫组织化学MaxVisionTM染色方法检测20例HNPCC及20例SCRC标本的凋亡相关蛋白因子配体(FasL)蛋白的表达及肿瘤浸润淋巴细胞(TIL)数量、分布情况。另选正常大肠黏膜20例作为对照。结果 HNPCC、SCRC癌细胞及正常大肠粘膜细胞FasL蛋白的表达阳性率分别为30%、70%、0%,3者比较差异有统计学意义(P<0.05);遗传性非息肉病性大肠癌TIL数量高于散发性大肠癌(P<0.05),且其分布与FasL蛋白的表达为负相关关系(r=-0.483,P<0.05)。结论 HNPCC癌细胞FasL蛋白相对低表达或缺失可能是TIL记数高于散发性大肠癌和较之预后较好的重要原因之一。Objective To investigate the possible cause of increased tumor-infiltrating lymphocyte（TIL） number in hereditary nonpolyposis colorectal cancer（HNPCC） compared with sporadic colorectal carcinoma（SCRC）.Methods The expression of FasL protein was detected by immunohistochemistry and the number and distribution of TIL were determined in 20 HNPCC specimens,20 SCRC specimens and 20 normal mucosal specimens.Results The positive expression rates of FasL protein were,respectively,30%,70% and 0% in HNPCC,SCRC and normal mucosa,with significant differences among the three tissues（P〈0.05）.Moreover,the number of TIL in HNPCC was obviously higher than that in SCRC（P〈0.05）,and the distribution of TIL was negatively correlated with the expression of FasL（r=-0.483,P〈0.05）.Conclusion Low expression or absence of FasL protein in HNPCC may be the possible cause of increased TIL number and good prognosis compared with SCRC.

关 键 词：遗传性非息肉病性大肠癌 散发性大肠癌 FASL 肿瘤浸润淋巴细胞 免疫逃逸 

分 类 号：R735.34[医药卫生—肿瘤]