盐酸美金刚对血管性痴呆大鼠疗效观察  

Effect of memantine on vascular dementia in rat

作　　者：孙治坤[1] 马兴荣[2] 杨红旗[3] 张杰文[1] 

机构地区：[1]河南省人民医院神经内科,郑州450003 [2]郑州大学第一附属医院神经内科,郑州450052 [3]河南省人民医院老年医学部神经内科,郑州450003

出　　处：《中国实用神经疾病杂志》2012年第13期1-4,共4页Chinese Journal of Practical Nervous Diseases

摘　　要：目的观察盐酸美金刚对血管性痴呆大鼠的疗效及作用机制。方法 80只健康Wistar大鼠(月龄12～14个月),体质量300～400g,随机分为对照组、模型组、美金刚对照组及美金刚治疗组,每组20只。采用持久双侧颈总动脉结扎术造成血管性痴呆大鼠模型,美金刚对照组及美金刚治疗组于术后8周开始以美金刚(5mg.kg-1)每天灌胃,对照组和模型组以同等量的0.5g/L羧甲基纤维素钠灌胃,连续4周。采用Morris水迷宫衡量大鼠学习记忆水平;测定大鼠脑皮层、海马组织乙酰胆碱酯酶(AChE)、胆碱乙酰转移酶(ChAT)、丙二醛(MDA)、谷胱甘肽(GSH)活性的变化。结果术后12周,与对照组相比,模型组大鼠的学习记忆能力明显下降(P<0.05);脑皮层、海马组织内AChE活性明显升高(P<0.05),ChAT活性明显降低(P<0.05),MDA活性明显升高(P<0.05),GSH活性明显降低(P<0.05);与模型组相比,美金刚治疗组大鼠的学习记忆能力明显提高(P<0.05);脑皮层、海马组织内AChE活性及ChAT活性差异无统计学意义(P>0.05)、MDA活性明显降低(P<0.05)、GSH活性明显升高(P<0.05)。结论盐酸美金刚对血管性痴呆大鼠的学习记忆能力有明显提高,作用机制是通过调节脑组织内MDA及GSH的活性来实现的,该实验研究为临床上血管性痴呆的治疗提供实验基础及理论依据。Objective To observe the effect of memantine on vascular dementia in rats. Methods Eighty healthy Wistar rats were randomly divided into control group, model group, the memantine group and memantine treatment group （n= 20）. Eight weeks after operation, memantine （5 mg. kg-1 ） was give to memantine group and memantine treatment group orally every day, and the same amount of sodium carboxymethylcellulose gavage was give to the control group and model group for 4 weeks. The water maze test was performed to examine the ability of learning and memory of the rats, and the activities of acetylcholinesterase （ACHE）, choline acetyhransferase （CHAT）, malondialdehyde （MDA）, giutathione （GSH） in rat cerebral cortex, hippocampus were also determined respectively. Results After 12 weeks, the learning and memory ability of the model group were significantly decreased compared with the control group （P〈0.05）. The activity of AChE was increased （P〈 0.05）, the activity of ChAT was decreased （P〈0.05）, the activity of MDA was increased （P〈0.05）, and the activity of GSH was decreased （P〈0.05）. Compared with model group, the learning and memory in the memantine treatment group were significantly increased （P〈0.05） ; The activity of MDA was decreased （P〈0.05）, and the activity of GSH was increased （P 〈0.05）. However, the activity of AChE and ChAT had no significantly difference（P〉0.05）. Conclusion Memantine can significantly improve the learning and memory ability of rats with vascular dementia, and its mechanism is achieved by regulating the activity of MDA and GSH.

关 键 词：血管性痴呆 盐酸美金刚 丙二醛 谷胱甘肽 

分 类 号：R-332[医药卫生]