环境水体中霍乱弧菌的二次PCR检测方法  

Twice PCR Detection Method of Vibrio cholera in Water

作　　者：黄海[1] 周登仁[1] 王琼妹[1] 邝仕壮[1] 庞燕[1] 

机构地区：[1]海口市疾病预防控制中心微生物检验科,海南571100

出　　处：《环境与健康杂志》2012年第7期643-645,共3页Journal of Environment and Health

摘　　要：目的建立环境水体中霍乱弧菌的二次PCR检测方法。方法对霍乱弧菌的外环境水监测样本进行PCR检测;确定弱电泳条带或无电泳条带的稀释度,将第一次PCR的产物梯度稀释后进行二次PCR。采用琼脂凝胶糖电泳进行分析。结果对378件环境水样分别进行一次、二次PCR检测,有4件水样经两种方法检测均为阳性,有6件水样二次PCR检测为阳性。一次PCR检测霍乱弧菌的阳性率[1.06%(4/378)]低于二次PCR[2.65%(10/378)],差异有统计学意义(P<0.05)。结论二次PCR操作简便快速、检出率高、费用低廉,适用于环境水样中霍乱弧菌的快速监测。Objective To establish the twice PCR detection method of Vibrio cholera in water. Methods The twice PCR was applied to detect Vibrio cholera in environmental water after the first product of PCR, then gel electrophoresis analysis was used. Results Four samples were detected to be positive by the two methods and six samples were positive by twice PCR. The positive rate of the once PCR was less than the twice PCR with significant difference （P〈O.05）. Conclusion The twice PCR is rapid, efficient, cheaper and applicable to the rapid detection of Vibrio cholera in the environmental water.

关 键 词：二次PCR 水 霍乱弧菌 

分 类 号：R117[医药卫生—公共卫生与预防医学]