人乳头状瘤病毒和EB病毒在子宫颈上皮内瘤变中的表达  

Expression of human papillomavirus and Epstein-Barr virus in cervical intraepithelial neoplasia

作　　者：钟辉[1] 刘锦钰[2] 龙璐雯 

机构地区：[1]安徽省淮北市妇幼保健院病理科,235000 [2]安徽省淮北市妇幼保健院妇科,235000

出　　处：《蚌埠医学院学报》2012年第7期765-768,共4页Journal of Bengbu Medical College

基　　金：安徽省淮北市科技局基金资助项目(201002043)

摘　　要：目的:探讨人乳头状瘤病毒(HPV)和EB病毒(EBV)在子宫颈上皮内瘤变(CIN)中的表达。方法:采用原位杂交对130例CIN(CIN1 50例,CIN2 40例,CIN3 40例)、子宫颈癌10例、子宫颈湿疣样病变10例、子宫颈正常黏膜10例的HPV(高危型16/18、低危型6/11)和EBV进行检测。结果:HPV16/18在CIN1、CIN2、CIN3的阳性表达率分别是44.0%、37.5%和62.5%,子宫颈癌为8/10,子宫颈湿疣样病变为10/10,子宫颈正常黏膜为3/10,差异有统计学意义(P<0.01)。HPV6/11在CIN1、CIN 2、CIN3的表达率分别是36.0%、52.5%和70.0%,在子宫颈癌中的表达是6/10,子宫颈湿疣样病变9/10,子宫颈正常黏膜3/10,差异有统计学意义(P<0.01)。HPV16/18和HPV6/11在CIN1、CIN2、CIN3中联合表达率分别为26.0%、22.5%和50.0%,子宫颈癌为6/10,子宫颈湿疣样病变为7/10,子宫颈正常黏膜为2/10,差异有统计学意义(P<0.01)。EBV在CIN1、CIN2、CIN3的阳性表达率分别是24.0%、12.5%和22.5%,子宫颈癌为3/10,子宫颈湿疣样病变为0/10,子宫颈正常黏膜为0/10,差异无统计学意义(P>0.05)。结论:HPV感染是引起CIN的主要致病因子,临床上往往是多种亚型混合感染。EB病毒在CIN和子宫颈癌表达的意义仍有争议。Objective： To explore the expression of human papillomavirus （HPV） and Epstein-Barr virus （EBV） in cervical intraepithelial neoplasia（CIN）. Methods：The high-risk HPV（16/18） ,low-risk HPV（6/ll ） and EBV of 130 cases of CIN（50 CIN1, 40 CIN2,40 CIN3 ） , 10 cases of invasive cervical carcinomas, 10 cervical condyloma-like lesions and 10 normal mucosa of the uterine cervix were detected using in situ hybridization（ISH） technique. Results：The positive expression rates of HPV16/18 in CIN1, CIN2 and CIN3 were 44.0%, 37.5% and 62.5%, respectively; and positive HPV16/18 was detected in 8/10 of the invasive cervical carcinomas, 10/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix （ P 〈 0.01）. The positive expression rates of HPV6/ll in CIN1, CIN2 and CIN3 were 36.0% ,52.5% and 70.0% , respectively; and positive HPV6/ll was detected in 6/10 of the invasive cervical carcinomas ,9/10 of cervical condyloma-like lesions and 3/10 of normal mucosa of the uterine cervix（P〈0.01）. The combined positive expression rates of HPV16/18 and HPV6/ll in CIN1, CIN2 and CIN3 were 26.0%, 22.5% ,and 50.0% ,respectively;and combined positive expression of HPV16/18 and HPV6/ll was detected in 6/10 of the invasive cervical carcinomas,7/10 of cervical condyloma-like lesions and 2/10 of normal mucosa of the uterine cervix（P 〈 0.01 ）. The positive expression rates of EBV in CIN1 ,CIN2 and CIN3 were 24.0%, 12.5% and 22.5% ,respectively;and EBV was detected positive in 3/10 of the invasive cervical carcinomas ,0/10 of cervical condyloma-like lesions and 0/10 of normal mucosa of the uterine cervix（ P 〉 0.05）. Conclusions： HPV infection is the major etiological factor for CIN, clinically often coinfected with some variants. The signification of positive expression of EBV in CINs and invasive cervical carcinomas is still debatable.

关 键 词：子宫颈肿瘤 子宫颈上皮内瘤变 人乳头状瘤病毒 EB病毒 病理学 临床 

分 类 号：R737.33[医药卫生—肿瘤]