胃癌凋亡相关蛋白Survivin、B淋巴细胞／白血病-2、bax表达与肿瘤细胞体外化疗药敏性的关系  

Relationship between expressions of apoptosis-related proteins and chemosensitivities in gastric cancer

作　　者：檀碧波[1] 李勇[1] 范立侨[1] 赵群[1] 刘羽[1] 赵雪峰[1] 

机构地区：[1]河北医科大学第四医院外三科,石家庄050011

出　　处：《中华实验外科杂志》2012年第7期1315-1317,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81072033）;河北省自然科学基金资助项目（C2010000619）;河北省普通高校强势特色学科资助项目[冀教高（2005）52];河北省卫生厅科研基金资助项目（20110460）

摘　　要：目的探讨胃癌凋亡相关蛋闩表达与体外化疗药敏性的关系。方法对64例胃癌标本进行Survivin、B淋巴细胞／白血病-2（bcl-2）、bax免疫组织化学染色，并以噻唑蓝（MTF）比色法检测体外化疗药物敏感性。结果肿瘤Smwivin、bcl-2、bax阳性表达率分别为90．6％、75．0％、68．8％；Survivin与bax、bcl-2与bax间表达强度均呈负相关（r=-0．45044、-0．41403，P〈0．01）。在耐药因子表达程度与药物对肿瘤细胞抑制率的关系中，Smwivin强表达时，表阿霉素（eADM）、顺铂（DDP）对肿瘤细胞的抑制率明显降低（P〈0．05），但奥沙利铂（L—OHP）对肿瘤细胞的抑制率明显增加（P〈0．05）；bcl-2强表达时，5-氟尿嘧啶（5-FIJ）、紫杉醇（PTX）、eADM对肿瘤细胞的抑制率明显低于弱表达组（P〈0．05）；bax强表达组中，5-Fu、eADM、L—OHP和甲氨喋呤（MTX）对肿瘤细胞的抑制率明显高于弱表达组（P〈0．05）。结论胃癌凋亡相关蛋白表达与部分化疗药物敏感性有关。Objective To investigate the relationship between expression of apoptosis-related pro- teins and chemosensitivities in gastric carcinoma. Methods The expression levels of Survivin, B lympho- eytes/leukemia-2 （bcl-2） and bax were determined immunohistochemically, and the chemosenisitivities of 9 drugs were measured by methyl thiazol tetrazolium （MTT） assay in 64 tissue specimens of gastric carci- nomas. Results The positive rate of Survivin, bcl-2 and bax was 90. 6% , 75.0% and 68.8% respective- ly. There was negatively significant correlations between the expression of Survivin and bax, and bel-2 and bax （r = -0. 450 44, -0. 414 03, both P〈0. 01 ）. In terms of relationship between the expression of Survivin, bcl-2, bax and inhibition rate of tumor cells, the inhibition rate for epirubicin （eADM） and cis- platin （DDP） in Survivin strong expression group was lower than that in weak group （both P 〈 0. 05 ）, but for oxaliplatin （L-OHP） the inhibition rate was increased （ P 〈 0. 05 ）. The inhibition rate to 5-fluorouraeil （5-Fu）, paclitaxel （PTX） and eADM was significantly lower in the bcl-2 strong expression group than the weak group （all P 〈 0. 05）. There was higher inhibition rate for 5-Fu, eADM, L-OHP and methotrexate （MTX） in bax strong expression group （ all P 〈 0. 05 ）. Conclusion The expression of apoptosis-related proteins in gastric carcinoma was associated with the chemosensitivities of parts of drugs.

关 键 词：胃肿瘤 多药耐药性 凋亡相关蛋白 体外药敏实验 

分 类 号：R735.2[医药卫生—肿瘤]