全反式维甲酸对人结肠癌细胞亚株SW480／M5增殖和凋亡的影响  

Ali-trans retinoic acid in human colorectal cancer cell subline SW480/M5 on growth and apoptosis

作　　者：吕会增[1] 李玺[2] 陈图峰[2] 叶小勇[1] 周如健[1] 

机构地区：[1]广州医学院第五附属医院普外科,510700 [2]中山大学第三附属医院普外科

出　　处：《中华实验外科杂志》2012年第7期1321-1323,共3页Chinese Journal of Experimental Surgery

基　　金：广州医学院博士启动基金资助项目（20100136）

摘　　要：目的观察全反式维甲酸（ATRA）对人结肠癌细胞亚株SW480／M5增殖和凋亡的影响。方法噻唑蓝（MTT）比色法检测1、2、4、8μmoL／LATRA作用24、48、72h对SW480／M5细胞的抑制率。流式细胞仪检测8μmoL／LATRA作用24、48、72h及2、4μmoL／LATRA作用48h的肿瘤细胞周期和凋亡率。结果ATRA抑制SW480／M5细胞增殖作用呈一定时效和量效依赖性；8μmoL／LATRA作用72h明显抑制肿瘤细胞增殖，抑制率接近30％。2、4μmoL／LATRA作用SW480／M5细胞48h或8μmoL／L作用24h，肿瘤细胞G，期比例开始降低，S期比例增加（P〈0．05）。8μmoL／LATRA作用48h后，S期比例进一步增高，G2／M期细胞比例不增加；作用72h后，G，期细胞比例进一步下降，伴G2／M期细胞比例增加（P〈0．05）。8μmoL／LATRA作用24h无明显诱导SW480／M5细胞凋亡作用，作用48h或72h可诱导肿瘤细胞凋亡，但凋亡率差异无统计学意义（P〉0．05）。结论8μmol／LATRA作用48h或72h通过阻滞SW480／M5细胞在S期或（和G2／M）期，并诱导肿瘤细胞凋亡，可明显抑制肿瘤细胞增殖。Objective To investigate roles of all-trans retinoic acid （ ATRA in human colorectal cancer cell subline SWdSO/M5. Methods The effect of ATRA at 1, 2, 4, 8 μmol/L for 24, 48, 72 h inhibiting cell growth by % in SW480/M5 cells was determined by methyl thiazol tetrazolium （MTT） as- say. The cell cycle and apoptosis induced by ATRA at 8 μmol/L for 24, 48, 72 h respectively or at 2, 4 IxmoL/L for 48 h was assessed by flow cytometry. Results In SW480/M5 cells, ATRA was a dose-and time-dependent drug, which had significant growth inhibitory activity nearly at 30% against SW680/M5 cells after a 72 h continuous treatment＇at 8 μmol/L. With ATRA at 2 or 4 μmol/L for 48 h or 8 μmol/L for 24 h in SW480/M5 cells, the percentage of cells in Gl phase was reduced and increased was that in S- phase （P 〈 0. 05）. With ATRA at 8 μmol/L in SW480/M5 cells for 48 h, the percentage of cells in GI phase was reduced, in addtion that in S-phase was increased significantly and that in GJM-phase was not increased than the wild cancer cells （ P 〈 0. 05 ） ; With ATRA at 8 μmol/L in SW480/M5 cells for 72h, the percentages of reduced cells in G1 -phase were more than that for 48h and the percentages of increased ceils in G2/M-phase were more than that of the wild cancer cells （P 〈0.05） ; With ATRA at 8 p.mol/L for 24 h in SW480/M5 cells, the ceils were not induced to apoptosis obviously. The apoptosis rate of SW480/ M5 cells induced significantly by ATRA at 8 μmol/L for 48h were just same as that for 72 h （ P 〉 0.05 ）. Conclusion ATRA at 8 μmol/L for 48h or 72h can significantly inhibit SW480/M5 cells growth by bloc- king the cells into S-phase or （and into GJM-phase） and inducing the cells apoptosis.

关 键 词：全反式维甲酸 人结肠癌细胞亚株 细胞周期 凋亡 

分 类 号：R735.35[医药卫生—肿瘤]