食管癌组织人乳头瘤病毒感染与人端粒酶RNA基因表达的关系  

Relationshap between human papUlomavirus DNA testing and human telomerase RNA gene amplifi- cation detection in esophageal cancer tissue

作　　者：王新帅[1] 陈强[1] 王玉峰[1] 高社干[1] 张广平[1] 冯笑山[1] 

机构地区：[1]河南科技大学第一附属医院肿瘤中心,洛阳471003

出　　处：《中华实验外科杂志》2012年第7期1355-1356,共2页Chinese Journal of Experimental Surgery

基　　金：河南省科技攻关重大项目（112101310100）

摘　　要：目的探讨食管癌组织中人乳头瘤病毒（HPV）感染与人端粒酶RNA（hTERC）基因表达的关系。方法82例经病理确诊的食管癌患者组织标本，聚合酶链反应（PCR）技术结合等离子谐振技术检测食管癌组织中HPV感染，荧光原位杂交技术检测食管癌组织中hTERC基因的表达。结果食管癌组织中存在HPV感染，感染率为43．7％，主要以混合型为主，包括高危亚型HPV16、18；食管癌组织中hTERC基因的表达率为80．1％；HPV感染与hTERC基因表达关系密切（x2=9．834，P〈0．05）。结论食管癌组织中HPV感染与hTERC基因表达是食管癌发病的重要因素．官可能通过hTERC基因途径引起痛变结果。Objective To study the relationshap between human papillomavirus （HPV） DNA tes- ting and human teiomerase （hTERC） RNA gene amplification detection in esophageal cancer tissue. Meth- ods HPV DNA was tested by polymerase chain reaction （PCR） in 82 cases of esophageal cancer tissues. The fluorescence signals of hTERC in the esophageal cancer were detected by using interphase fluorescence in situ hybridization （FISH）. Results There was HPV infection in esophageal cancer tissue, and the in- fection rate was about 43.7%. Mixed type was predominant, including the HPV 16 and 18. The mutation rate of hTERC in esophageal cancer tissue was about 80. 1%, and there was a positive correlation between the HPV infection and mutation of hTERC （ P 〈 0. 05 ）. Conclusion HPV infection and mutation of hTERC in the esophageal cancer may be important factors of pathogenesis. The mutation of hTERC gene may play an important role in the development of sequamous cell carcinoma.

关 键 词：食管癌 乳头瘤病毒 染色体端粒酶基因 

分 类 号：R735[医药卫生—肿瘤]