^(16)O^(6+)辐照诱导的人肝癌细胞DNA双链断裂及其修复  

INDUCTION AND REPAIR OF DNA DOUBLE-STRAND BREAKS IN HUMAN HEPATOMA CELLS IRRADIATED WITH ^(16)O^(6+)

作　　者：周光明[1] 卫增泉 李文建[1] 王菊芳[1] 何静[1] 李强[1] 党秉荣[1] 颉红梅[1] 高清祥[2] 

机构地区：[1]中国科学院近代物理研究所,兰州730000 [2]兰州大学生物系,兰州730000

出　　处：《辐射研究与辐射工艺学报》2000年第3期198-202,共5页Journal of Radiation Research and Radiation Processing

基　　金："西部之光"资助!(XB980604)

摘　　要：用倒转脉冲场凝胶电泳（ＰＩＧＥ） 研究了 ３．１７ＭｅＶ／ｕ１６Ｏ６＋ 诱导的肝癌细胞 ＤＮＡ双链断裂（ＤＳＢ）及其修复效应。结果表明： ＤＳＢ的诱导与辐照剂量呈正相关，其产额为 ０．４３ＤＳＢｓ／１００Ｍｂｐ：Ｇｙ，与 ６０Ｃｏγ射线相比相对生物学效应为 １．６９。 ＤＳＢ片段重接一半所需要的时间（ｔ１／２）与剂量有关，剂量越大，ｔ１／２越长。重接的方式主要表现为小片段连接为较大的片段。Pulsed-field gel electrophoresis has been applied to analyse the induction and repair of DNA double-strand breaks (DSB) in human hepatoma SMMC-7721 cells exposed to 3.17MeV/u 16O6+. It shows that the DSB yields are 0.43DSBs/100Mbp/Gy and compared with 60Co γ-rays , the relative biological effectiveness (RBE) of 16O6+ is 1.69. The half time of DSB fragments being repaired (t1/2) is relevant to the dose; the higher the dose is, the longer the time is. The main way for DSB repair is the rejoining of smaller fragments into larger ones.

关 键 词：^16O^6+ DNA双链断裂 人肝癌细胞 辐照诱导 

分 类 号：Q691.5[生物学—生物物理学] R735.7[医药卫生—肿瘤]