甲状腺乳头状癌中XAFl基因启动子甲基化及其蛋白表达的相关性  

Relationship between methylation status of promoter and expression of XAF1 gene in papillarythyroid carcinoma

作　　者：殷德涛[1] 陈国[1] 李红强[1] 郑湘予[2] 王勇飞[1] 张艳 江金花 王庆端 

机构地区：[1]郑州大学第一附属医院甲状腺外科河南省高等学校临床医学重点学科开放实验室,郑州450052 [2]郑州大学第一附属医院病理科,郑州450052 [3]河南省医药科学研究院

出　　处：《中华医学杂志》2012年第28期1967-1970,共4页National Medical Journal of China

基　　金：河南省卫生科技创新型人才工程专项基金（201052）

摘　　要：目的探讨甲状腺乳头状癌（PTC）组织中x染色体连锁凋亡抑制因子相关因子1（XAFl）基因启动子甲基化及其与蛋白表达的相关性。方法采用甲基化特异性聚合酶链反应（PCR，MSP）及免疫组化（sP法）检测70例PTC及其对应的癌旁非癌组织（NCE）中XAFl基因启动子甲基化及其蛋白的表达。结果在NCE中，无一例出现XAFl基因启动子甲基化，而在PTC组织中有35．7％（25／70）出现甲基化（x^2=27．206，P〈0．01），且甲基化状态与PTC的TNM分期、病理分级及淋巴结转移有关（P〈0．05）；NCE和PTC中，XAFl蛋白表达的阳性率分别为100％（70／70）和55．7％（39／70）（）（。：36．458，P〈0．01）。在PTC中，病理分级I级和Ⅱ级组，XAFl蛋白阳性率分别为67．5％（27／40）和40．0％（12／30），在淋巴结转移阳性和阴性组中分别为35．7％（10／28）和69．0％（29／42），以上两组比较差异有统计学意义（x^2值分别为5．254和7．565，P〈0．05）；XAFl基因启动子甲基化与其蛋白表达有明显的相关性（x^2=8．864，P〈0．01）。结论XAFl基因启动子甲基化是基因失活的重要机制之一，在PTC的发生、发展中起着重要的作用。Objective To explore the relationship between methylation status of XAF1 （X-linked inhibitor of apoptosis protein associated factor-1 ） gene promoter and its protein expression in papillary thyroid carcinoma （PTC） . Methods Methylation-speeific polymerase chain reaction （MSP） and immunohistochemical substance P （SP） technique were used to detect the methylation status of XAF1 gene promoter and its protein expression in 70 PTC cases and their matched adjacent non-cancerous epithelium （NCE）. Results In NCE, there was no promoter methylation of XAF1 gene while the rate was 35.7% （25/70） in PTC （ x^2= 27. 206, P 〈 0. 01 ）. And it was correlated with tumor TNM stage, pathological grade and lymph node metastasis （P 〈 0.05）. The positive rates of XAF1 protein expression in NCE and PTC were 100% （70/70） and 55.7% （39/70） respectively. And there was significant difference（x^2= 36. 458, P 〈0.01 ）. In PTC, the positive rates of XAF1 protein expression in Grades I and Ⅱwere 67.5% （27/40） and 40.0% （12/30） respectively. And they were 35.7% （10/28） and 69. 0% （29/42） in the lymph node metastasis and non-metastasis groups respectively. And there were significant differences between two groups （P 〈 0. 05 ）. Futhermore, there was distinct correlation between methylation of XAF1 gene promoter and its protein expression （x^2= 8. 864, P 〈 O. 01 ）. Conclusion Methylation of promoter may be one of the important inactivating factors of XAF1 gene. And it plays an important role in the carcinogenesis and progression of PTC.

关 键 词：甲状腺肿瘤 甲基化 免疫组织化学 X染色体 细胞凋亡 

分 类 号：R736.1[医药卫生—肿瘤]