ox-LDL对巨噬细胞内皮脂肪酶表达的影响  

ox-LDL increases endothelial lipase in RAW264.7 macrophages

作　　者：吴晓倩[1] 宜全[1] 何丽姗[1] 

机构地区：[1]广州医学院药理教研室,广东广州510182

出　　处：《中国病理生理杂志》2012年第7期1172-1175,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81001436);广东省自然科学基金资助项目(No.10451018201005279);广州市首届"羊城学者"科研资助计划青年科研骨干培养项目(No.10A018G);广东省高校优秀青年创新人才培养项目(No.LYM10110);广州市属高校科研项目(No.10A157)

摘　　要：目的:探讨氧化低密度脂蛋白(ox-LDL)对鼠源RAW264.7巨噬细胞内皮脂肪酶(EL)表达的影响。方法:用ox-LDL(0～100 mg/L)孵育RAW264.7细胞24 h使其活化,或者用PDTC(NF-κB抑制剂)预孵育RAW264.7细胞30 min再与ox-LDL(50 mg/L)共同孵育24 h;应用Western blotting检测EL和NF-κB p65的表达。结果:ox-LDL孵育RAW264.7细胞明显增加EL表达(P<0.05);ox-LDL(50 mg/L)孵育RAW264.7细胞15～30 min可激活NF-κB,用NF-κB抑制剂PDTC处理后则明显抑制ox-LDL诱导的EL表达增加(P<0.05)。结论:ox-LDL可明显增加RAW264.7细胞EL的表达,可能与NF-κB活化有关。AIM： To investigate the effect of oxidized low - density lipoprotein （ ox - LDL） on the expression of endothelial lipase （ EL ） in routine BAW264.7 maerophages. METHODS： RAW264. 7 cells were incubated with ox - LDL at concentrations of 0 - 100 mg/L for 24 h. On the other hand, the cells were incubated with or without PDTC （ NF - KB inhibitor） for 30 min and then with ox - LDL （50 mg/L） for 24 h. The expression of EL and p65 was detected by Western blotting. RESULTS： The level of EL was significantly increased after ox - LDL incubation in RAW264.7 cells （P〈0.05）. NF - KB was activated by ox -LDL at concentration orS0 mg/L for 15 -30 w.in in RAW264.7 cells. The in- crease in EL induced by ox - LDL was markedly inhibited by a NF - KB inhibitor PDTC （ P 〈 0.05 ）. CONCLUSION： ox - LDL significantly increases the expression of EL in RAW264.7 macrophages, which is possibly related to NF - KB acti- vation.

关 键 词：内皮脂肪酶 NF—κB 氧化低密度脂蛋白 胆固醇 HDL 

分 类 号：R363[医药卫生—病理学]