PMA特异性抑制K562细胞BCR/ABL和Fyn mRNA的表达  

PMA specifically inhibits mRNA expression of BCR/ABL and Fyn in K562 cells

作　　者：王冠明[1] 陈小华[1] 林晨[1] 陈少华[2] 杨力建[2] 王鹏程[1] 李扬秋[2] 

机构地区：[1]暨南大学医学院微生物与免疫学系,广东广州510632 [2]暨南大学血液研究所,广东广州510632

出　　处：《中国病理生理杂志》2012年第7期1258-1261,共4页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.06025169);广东省自然科学基金博士科研启动项目(No.10451063201005505);广州市科技支撑计划项目(No.2009Z1-E161);广东省卫生厅医学科研基金资助项目(No.A2010313)

摘　　要：目的:研究12-肉豆蔻酸-13-乙酸佛波酯(PMA)对K562细胞BCR/ABL与Fyn mRNA表达的影响及两者之间的关系。方法:1～250μg/L PMA刺激K562细胞24 h后,应用实时荧光定量PCR技术检测各组BCR/ABL和Fyn mRNA的水平。结果:PMA明显抑制BCR/ABL和Fyn mRNA的水平,该下调作用均具有显著的量效关系,且BCR/ABL和Fyn的mRNA表达水平下调表现出明显的相关性。比较刺激Molt-4细胞株的结果,PMA抑制作用具有细胞选择性。K562细胞株经诱导后出现伪足样的形态改变。结论:PMA通过抑制BCR/ABL融合基因的转录下调Fyn转录水平。AIM： To study the effects of pborbol 12 - myristate 13 - acetate （PMA） on the mRNA expression of BCR/ABL and Fyn in K562 cells, and to explore their relationship. METHODS： The K562 cells were stimulated by PMA at a series of concentrations （1 - 250 μg/L） for 24 h, and the mBNA expression levels of BCR/ABL and Fyn in K562 cells were detected by real - time fluorescence quantitative PCR . The relative changes of both mRNA expression were measured using 2- aact formula. RESULTS： PMA significantly inhibits the mRNA levels of BCR/ABL and Fyn in a dose- dependent manner, and the correlation of these inhibitory effects were significant. Compared with gene Molt- 4 cells, the inhibition by PMA was specific for KS62 cells. The K562 cells were induced to differentiate to be pseudopedium -like cells. CONCLUSION： The PMA downregulates the mRNA level of Fyn by inhibiting BCR/ABL fusion gene.

关 键 词：12-肉豆蔻酸-13-乙酸佛波酯 Fyn蛋白 融合蛋白 BCR—ABL 

分 类 号：R364.14[医药卫生—病理学]