柯萨奇B4病毒5'UTR基因序列及遗传进化分析  

5′UTR Sequence and phylogenetic analysis of a coxsackievirus B4

作　　者：周密[1] 李凡[2] 王艳艳[3] 

机构地区：[1]长春医学高等专科学校,吉林长春130031 [2]吉林大学白求恩医学院 [3]吉林大学第一医院儿科

出　　处：《中国实验诊断学》2012年第7期1160-1162,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省教育厅"十一五"科学技术研究项目资助(2009551)

摘　　要：目的研究柯萨奇B4病毒(CVB4jlu06株)5′UTR基因序列及变异性,探讨其致病性的分子生物学基础。方法 Hep-2细胞扩增病毒,RT-PCR扩增5′UTR基因片段,测序后进行同源性、变异性及遗传进化分析。结果 CVB4jlu06株5′UTR基因全长743个核苷酸,其与Tuscany株和JVB株有同源性较高,遗传距离较近;CVB4jlu06株5′UTR基因与CVB4致糖尿病株(E2株、E2b株和Tuscany株)有三个共同核苷酸。结论 CVB4jlu06株5′UTR基因序列与CVB4JVB株和CVB4Tuscany亲缘关系密切;CVB4jlu06株5′UTR发生的核苷酸变异可能与其致病性相关。Objective To study the 5′UTR nucleotide sequence and variation of Coxsackievirus B4 jlu06 strain,and characterize the genetic determinants involved in pathogenic phenotype.Methods Coxsackievirus B4 was amplified with Hep-2 cell.The 5′UTR gene fragment was amplified by RT-PCR and sequenced,the mutations,homology and phylogenesis were analyzed.Results The 5′UTR of the jlu06 strain is 743 nucleotides（nt） long,which showed high homologies and short genetic distance to Tuscany strain and JVB strain.The 5′UTR nucleotide sequence of CVB4 jlu06 strain showed common amino acids with diabetogenic strains（E2,E2b and Tuscany strains） at 3 sites.Conclusion The 5′UTR of the jlu06 strain is closely related to CVB4Tuscany and CVB4JVB strain;The mutations in 5′UTR nucleotide sequence might be responsible for the pathogenic phenotype of this strain.

关 键 词：柯萨奇病毒 核苷酸序列 遗传进化 

分 类 号：R373[医药卫生—病原生物学]