纳米Fe_3O_4/CD133Ab复合物对胶质瘤U251细胞放疗增敏作用的实验研究  

Experimental study on radiosensitization effect of nano Fe_3O_4/CD133Ab complex on human brain glioma U251 cells

作　　者：袁喜[1] 孙新臣[2] 赵迪 孟庆红[1] 马珺 童金龙 

机构地区：[1]东南大学附属中大医院肿瘤科,南京210009 [2]江苏省人民医院放疗科,210029 [3]南京市第二人民医院肿瘤科,210003

出　　处：《临床肿瘤学杂志》2012年第7期577-581,共5页Chinese Clinical Oncology

基　　金：国家自然科学基金资助项目(30970792)

摘　　要：目的探讨纳米Fe3O4/CD133Ab复合物对脑胶质瘤U251细胞的放射增敏作用。方法细胞免疫组化法检测脑胶质瘤U251细胞CD133的表达情况;流式细胞分选获得U251-CD133+细胞;MTT法观察纳米Fe3O4/CD133Ab复合物和CD133Ab分别对脑胶质瘤细胞U251及U251-CD133+的细胞毒作用;克隆形成抑制实验检测纳米Fe3O4/CD133Ab复合物和CD133Ab对脑胶质瘤U251细胞放射增敏的影响。结果脑胶质瘤U251细胞中有CD133分子阳性表达并可以分选出CD133阳性表达细胞;在0.0488～25μg/ml范围内,纳米Fe3O4/CD133Ab复合物作用于脑胶质瘤U251及U251-CD133+细胞24h的细胞毒性呈明显剂量依赖性,与阴性对照组比较,差异有统计学意义(P>0.05)。纳米Fe3O4/CD133Ab复合物对脑胶质瘤U251和U251-CD133+细胞的增殖抑制率最高值分别为59.46%和56.66%,IC50分别为11.84μg/ml和12.06μg/ml。纳米Fe3O4/CD133Ab复合物+照射组与CD133Ab+照射组比较,细胞存活分数差异有统计学意义(P<0.05),在照射4Gy以上时,随X射线剂量增加而放射敏感性增强。结论纳米Fe3O4/CD133Ab复合物对脑胶质瘤细胞U251和U251-CD133+有细胞毒作用,并且对U251细胞有放射增敏作用。Objective To investigate the radiosensitivity enhancement of nano Fe3O4/CD133Ab complex on human brain glioma U251 cells.Methods Immunohistochemical staining was used to detect the expression of CD133 molecules in human glioma U251 cells.U251-CD133＋cells were isolated by FACS.The toxicity of nano Fe3O4/CD133Ab complex and CD133Ab were determined by MTT.Colony-forming assay was employed to observe the effects of nano Fe3O4/CD133Ab complex and CD133Ab on the radiosensitivity of the human brain glioma U251 cells.Results The positive expression of CD133 molecules was observed in human glioma U251 cells,and CD133-positive cells were selected.The proliferation inhibition rate of human glioma U251 cells and U251-CD133＋cells gradually increased with the increasing concentration of nano Fe3O4/CD133Ab complex in 0.0488-25μg/ml range in a significant dose-effect manner.The maximum of tumor cell proliferation rate was 59.46% and 56.66%.The IC50 was 11.84μg/ml and 12.06μg/ml.The proliferation rate between nano Fe3O4/CD133Ab complex ＋ radiotherapy and CD133Ab＋ radiotherapy was of statistic significance（P〈0.05）.And when the X-ray doses were greater than 4Gy,the effects were enhanced with the increasing doses.Conclusion The nano Fe3O4/CD133Ab complex has toxicity on the human glioma U251 and U251-CD133＋cells.It also has radio-sensitizing effects on U251 cells

关 键 词：纳米Fe3O4/CD133Ab复合物 人脑胶质瘤U251细胞 放疗增敏 

分 类 号：R730.264[医药卫生—肿瘤]