胞磷胆碱对大鼠脊髓损伤的神经保护性作用  

Evaluation of the Neuroprotective Effects of Citicoline on Spinal Cord Injury of Rats

作　　者：王威[1] 郭风劲[2] 廖苏平[1] 危蕾[1] 吴波[1] 刘俊[1] 徐卫国[2] 

机构地区：[1]华中科技大学同济医学院附属普爱医院骨科,武汉430033 [2]华中科技大学同济医学院附属同济医院骨科,武汉430030

出　　处：《神经损伤与功能重建》2012年第4期258-262,共5页Neural Injury and Functional Reconstruction

摘　　要：目的:观察胞磷胆碱对大鼠脊髓损伤(SCI)的神经保护作用。方法:成年SD大鼠72只随机分为对照组、甲基强的松龙(MP)组、胞磷胆碱组及联合组,各组18只;造模后即刻腹腔注射给药1次,对照组给予等量生理盐水,MP组给予30mg/kg MP,胞磷胆碱组给予40mg/kg胞磷胆碱,联合组给予30mg/kg MP及40mg/kg胞磷胆碱;伤后8、24及72h每组各处死6只大鼠取材;HE染色观察脊髓组织的病理学变化,免疫组织化学方法观察半胱天冬氨酸酶(Caspase)-3和环氧化酶(COX)-2表达变化情况,DNA原位末端缺口标记法(TUNEL)检测神经细胞凋亡。结果:与对照组相比,MP组、胞磷胆碱组及联合组脊髓组织病理学改变明显减轻,Caspase-3和COX-2表达降低(P<0.05),凋亡细胞减少(P<0.05),MP组、胞磷胆碱组及联合组各组之间差异无统计学意义。结论:胞磷胆碱对大鼠SCI有神经保护性作用,与MP疗效相似,两者的联合应用与单独用药相比未见明显优势。Objective： To investigate the neuroprotective effects of citicoline on spinal cord injury （SCI） of rats. Methods. Seventy-two adult SD rats were randomly divided into four groups （n=18 respectively）： the control group （intraperitoneal injection of equal volume of normal sa- line）, the methylprednisolone （MP） group （intraperitoneal injection of 30 mg/kg MP）, the citi eoline group （intraperitoneal injection of 40 mg/kg citicoline） and the combined treatment group （intraperitoneal injection of 30 mg/kg MP and 40 mg/kg citicoline）. The rats were harvested at 8 h, 24 h, and 72 h （n=6 every time point） after SCI surgery. The tissue sections of the injured spinal cord were stained with hematoxylin and eosin （HE） to evaluate the histopathological chan- ges. Immunohistochemistry was applied to detect the expressions of Caspase-3 and COX-2 while TUNEL staining was performed to evaluate the apoptosis of cells. Results： When compared with that in the control group, the histopathologieal changes were attenuated, the expressions of Caspase-3 and COX-2 were down-regulated （P〈0.05） and the rate of cell apoptosis were decreased （P〈0.05） in the other three groups. But there were no statistical differences among the three groups. Conclusion： Citicoline has a similar efficacy as MP in treating SCI of rats. The effi- cacy of citicoline combined with MP does not show any synergistic effect.

关 键 词：脊髓损伤 胞磷胆碱 CASPASE-3 COX-2 

分 类 号：R741[医药卫生—神经病学与精神病学] R744[医药卫生—临床医学]