采用微量热法研究重金属离子对脲酶催化水解反应的影响  

Urease-catalyzed Reactions Inhibited by Heavy Metals Ion with Micro-calorimetry

作　　者：王丽萍[1] 胥义[1] 郑艺华[2] 李代禧[1] 徐斐[1] 

机构地区：[1]上海理工大学生物系统热科学研究所,上海200093 [2]青岛大学机电工程学院生物系统热科学研究所,青岛266071

出　　处：《高等学校化学学报》2012年第8期1771-1776,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50906056;50806036);上海市教委创新项目(批准号:11YZ111);上海市科委启明星项目(批准号:08QA14052)资助

摘　　要：利用微量热计Micro DSCⅢ对重金属离子影响脲酶催化水解反应的过程进行研究,并探讨重金属离子联合抑制作用对脲酶催化反应的影响.结果表明,在25℃下,重金属离子对脲酶水解反应热有影响,在广泛浓度范围(0.1,1,10,100和103mg/L)内,这种影响不仅表现在抑制作用方面,而且会促进其水解反应的进行,如铜离子在100和1000 mg/L时抑制率分别为(0.49±0.20)%和(-11.93±1.34)%.重金属离子浓度与抑制率具有显著或极显著的相关性,抑制效果的顺序为砷离子>铅离子>镉离子>铜离子.当重金属离子联合抑制时,其抑制效果与抑制率较高的重金属离子的抑制作用接近.Inhibiting effects of heavy metal ions on urease-catalyzed reaction were investigated with microcalorimetry. Further, the joint inhibition effects of two heavy metal ions on urease-catalyzed reaction were also conducted. The results indicate that, with a wide range of concentration（0. 1, 1, 10, 100 and 103 mg/L）, heavy metal ions not only inhibit urease hydrolysis reaction, but also can promote this reaction in some concentration range. For instance, inhibition rate is （0. 49±0.20） % and （-11.93±1.34） % when Cu2＋concentration is 100 and 1000 mg/L, respectively. The correlation between urease inhibition rate and concentration of heavy metal ion is very significant, and the inhibition effects are： As3＋ 〉Pb2＋ 〉Cd2＋ 〉Cu2＋. The joint inhibition is close to that with higher inhibition when two heavy metal ions are used together. This work proves that the micro-calorimetry may be a potential method to rapidly and accurately detect heavy metals.

关 键 词：微量热法 酶抑制法 重金属离子 抑制率 联合抑制作用 

分 类 号：O642.3[理学—物理化学]